黄瓜果实苦味(Bt)基因的插入缺失(Indel)标记  

The Insertion-deletion(Indel) Marker Linked to the Fruit Bitterness Gene(Bt) in Cucumber

作　　者：张圣平[1] 苗晗[1] 程周超[1] 张忠华[1] 武剑[1] 孙日飞[1] 顾兴芳[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《农业生物技术学报》2011年第4期649-653,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30900989);国家现代农业产业技术体系项目(No.Nycytx-35);农业部"948"研究项目(No.2008-Z42);中央级科研院所基本科研业务费专项(No.201011);农业部园艺作物遗传改良重点开放实验室共同资助

摘　　要：为了筛选与黄瓜果实苦味(Bt)基因紧密连锁的插入缺失(Indel)标记,本研究以黄瓜(Cucumis sativus L.)果实苦味和果实不苦的高代纯合自交系46GBt和931为亲本构建的F2分离群体为试材,明确了黄瓜果实苦味性状遗传是符合单显性基因控制的质量性状。同时在完成Bt基因初步定位和双亲重测序的基础上,利用生物信息学分析双亲在初步定位区域的序列差异,设计了7对InDel标记。通过对含有184个单株的F2群体分析,获得了与Bt基因连锁距离为0.8cM的Indel标记Bt-InDel-1。利用不同于本研究且包含58个单株的黄瓜F2群体材料对Bt-InDel-1的验证分析表明,该标记检测的正确率达到94.8%。本研究结果可用于无苦味黄瓜的分子标记辅助选择(MAS)育种及Bt基因精细定位。To identify Insertion-deletion（Indel） markers tightly linked to the fruit bitterness（Bt） gene in cucumber（Cucumis sativus L.）,a F2 population including 184 individuals derived from two cucumber inbred lines 931（btbt） and 46GBt（BtBt） was used in this study.Results showed that the inheritance of fruit bitterness in cucumber was controlled by one dominant gene Bt.Based on primary mapping of Bt gene and re-sequence of two parental lines,Bioinformatics was employed to analyze the sequence difference between the two parental lines in the primary mapping region of Bt gene.Then seven Indel markers were developed.The InDel marker Bt-InDel-1 tightly linked to Bt gene with 0.8 cM was identified after analysis on F2 population with 184 individuals using the seven Indel markers.The veracity of Bt-InDel-1 was tested using another F2 population with 58 individuals,and the accuracy rate for this marker was 94.8%.The results indicated that the Bt-InDel-1 marker can be used in non-bitter MAS cucumber breeding and fine-mapping of Bt gene.

关 键 词：黄瓜 果实苦味Bt基因 INDEL标记 

分 类 号：S511[农业科学—作物学]