山奈酚诱导人食管鳞癌Eca-109细胞凋亡及其机制  

Kaempferol-induced apoptosis of human esophageal squamous carcinoma Eca-109 cells and the mechanism

作　　者：李瑞君[1] 梅家转[1] 刘桂举 

机构地区：[1]郑州人民医院肿瘤内科,河南郑州450003

出　　处：《南方医科大学学报》2011年第8期1440-1442,共3页Journal of Southern Medical University

基　　金：郑州市博士创业基金(郑卫2009175)

摘　　要：目的研究山奈酚对人食管鳞癌Eca-109细胞增殖和凋亡的影响,并探讨其机制。方法山奈酚体外作用于Eca-109细胞后,用MTT法检测细胞增殖抑制作用;TUNEL染色测定细胞凋亡率;RT-PCR检测Bax、Bcl-2基因表达变化情况;分光光度法测定Caspase-3和Caspase-9活性。结果山奈酚显著抑制Eca-109细胞增殖(P<0.01),呈剂量依赖关系。TUNEL染色结果表明山奈酚诱导Eca-109细胞发生凋亡,RT-PCR结果显示山奈酚作用后,肿瘤细胞Bax基因表达上调,Bcl-2基因表达下调,同时,Caspase-3和Caspase-9活性明显升高(P<0.01)。结论山奈酚能抑制Eca-109细胞增殖,诱导细胞凋亡。山奈酚可能通过线粒体途径诱导Eca-109细胞凋亡。Objective To evaluate the growth-inhibiting and pro-apoptotic effect of kaempferol in human esophageal squamous carcinoma Eca-109 cells and explore the mechanism.Method The effect of kaempferol on Eca-109 cell proliferation in vitro was measured by MTT assay.TUNEL staining was used to detect the cell apoptosis following kaempferol treatment.The changes in Bax and Bcl-2 mRNA expressions in response to kaempferol treatment were determined by RT-PCR,and the caspase-3 and caspase-9 activities were evaluated using colorimetric assay.Results Kaempferol significantly inhibited Eca-109 cell proliferation（P0.05） in a concentration-dependent manner and induced obvious cell apoptosis.RT-PCR showed that after kaempferol treatment caused up-regulated Bax and down-regulated Bcl-2 mRNA expression.The colorimetric assay revealed significantly increased caspase-3 and caspase-9 activities in Eca-109 cells following kaempferol treatment（P0.01）.Conclusion Kaempferol can induce apoptosis of Eca-109 cells via a mitochondria-dependent pathway.

关 键 词：山奈酚 食管癌 细胞凋亡 ECA-109细胞株 

分 类 号：R735.1[医药卫生—肿瘤]