肿节风对人前列腺癌DU-145细胞PI3K／Akt／mTOR信号传导通路的影响  

Inhibition of PI3K/Akt/mTOR signal pathway in human prostatic cancer DU-145 cells treated with Sarcandra Glaba

作　　者：周仕轶[1] 王林[2] 丁维俊[3] 刘伟伟[3] 张蜀武[4] 

机构地区：[1]成都中医药大学临床医学院,成都611137 [2]邛崃市医疗中心医院 [3]成都中医药大学基础医学院 [4]成都中医药大学附属医院

出　　处：《中国男科学杂志》2011年第6期12-17,共6页Chinese Journal of Andrology

基　　金：成都中医药大学2010年校基金自然科学课题（编号：ZRYB201050）

摘　　要：目的探讨中药肿节风溶液对人前列腺癌DU-145细胞P13K／Akt/mTOR信号传导通路的影响。方法采用细胞培养的方法，用不同浓度的肿节风溶液作用于DU-145细胞48h，用MTT法检测肿节风溶液对DU-145细胞增殖的影响，用RT-PCR检测用药后细胞P13K、Akt、mTOR和P70^s6x的基因表达情况。结果肿节风溶液作用48h后，DU-145细胞增殖受到抑制，并表现为量效关系；DU-145细胞的P13K、Akt、mTOR和P70^s6k的表达均呈不同程度的抑制，但不表现为量效关系。结论肿节风溶液能抑制人前列腺癌DU-145细胞增殖，其抑制细胞增殖的机制与其抑制了细胞的P13K／Akt／mTOR信号传导通路的作用有关。Objective To investigate the effect of Sarcandra Glaba on PI3K/Akt/mTOR signal pathway in human prostatic cancer DU-145 cells. Methods DU-145 cells were treated with different concentration of Sarcandra Glaba solution for 48h. Proliferation of the treated DU-145 cells was measured by MTT. The expressions of PI3K, Akt, mTOR and P70^s6k were detected by RT-PCR. Results The growth of DU-145 ceils was inhibited and showed dosedependent effect. The expressions of PI3K, Akt, mTOR and P70^s6k were all downregulated. Conclusion Sarcandra Glaba might inhibit the growth of DU-145 cellsby regulating PI3K/Akt/mTOR signal pathway.

关 键 词：肿节风 DU-145细胞 增殖 PI3K/Akt/mTOR信号传导通路 

分 类 号：R737.25[医药卫生—肿瘤]