商南茶叶中微量硒的提取与测定  

Extraction and determination of trace selenium in Shangnan tea

作　　者：王书民[1,2] 樊雪梅[2] 陈凤英[2] 刘萍[2] 王宪生[2] 郑行望[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,西安710062 [2]商洛学院化学与化学工程系,商洛726000

出　　处：《分析仪器》2011年第4期57-59,共3页Analytical Instrumentation

基　　金：国家自然科学基金资助项目(20575040);商洛学院资助项目(10SKY015)

摘　　要：采用正交设计[L16(45)]试验,研究了茶叶中微量硒的提取和测定条件。在此基础上建立了铁氰化钾-盐酸存在下用微波消解-流动注射-氢化物发生-原子荧光光度法测定茶叶中微量硒的新方法。该方法的回归方程为y=62.91x-0.3529,R=0.9996,线性范围为0～20μg/L,检出限为0.01μg/L,相对标准偏差为2.7%(n=11)。用此方法测定了5种商南茶叶中的微量硒,回收率96.5%～103.4%,相对标准偏差2.9%～4.1%(n=7),结果令人满意。The experimental conditions for the extraction and determination of trace selenium in Shangnan tea were investigated using orthogonal design [L16（45）].Based on this,a microwave digestion-flow injection-hydride generation-atomic fluorescence spectrometric method has been developed for the determination of trace selenium in tea in the presence of ferricyanide potassium and hydrochloric acid.The regression equation of the method was y=62.91x0.3529（R=0.9996）.The linear range was 0-20μg/L with the detection limit of 0.01μg/L and the RSD of 2.7（n=11）.The trace selenium in 5 types of Shangnan tea were determined.The recoveries were 96.5%~103.4%,and the RSDs were 2.9%~4.1%（n=7）.The results were satisfactory.

关 键 词：微波消解 流动注射 氢化物发生 原子荧光光度法 茶叶 硒 

分 类 号：S571.1[农业科学—茶叶生产加工]