STAT3反义寡核苷酸纳米复合微粒的制备及特性研究  

Preparation and characterization of polyamidoamine dendrimer-STAT3-antisense oligodeoxynucleotides nanoparticles

作　　者：董勤[1] 崔杰[1] 高烽[1] 章菲菲[1] 米丽[2] 祝晴晴 

机构地区：[1]武警上海总队医院肾脏内科,上海201103 [2]武警上海总队医院药剂科,上海201103

出　　处：《武警医学院学报》2011年第9期723-725,728,共4页Acta Academiae Medicinae CPAPF

摘　　要：【目的】研究STAT3反义寡核苷酸纳米复合微粒的制备工艺，并对纳米复合微粒特性进行评价。【方法】将纳米技术与反义寡核苷酸技术相结合，制备STAT3反义寡核苷酸纳米复合微粒，并观察纳米微粒对限制性内切酶的抗性及其在不同pH值下的稳定性。【结果】将纳米技术与反义寡核苷酸技术相结合，制备STAT3反义寡核苷酸纳米复合微粒，并观察纳米微粒对限制性内切酶的抗性及其在不同pH值下的稳定性。【结果】在pH值为2～10的范围内，PAMAM—STAT3一asODN纳米微粒均未出现降解，且该纳米微粒具有较好的抗限制性内切酶的特性。【结论】新型STAT3反义寡核苷酸纳米微粒具有较好的抗限制性内切酶、耐酸碱的特性，为其在基因治疗中的应用提供了一定的依据。[Objective] To study the technique for the preparation of polyamidoamine dendrimer-STAT3-antisense oligodeoxy-nucleotides nanoparticles （PAMAM- STAT3- asODN- NPs）, and to evaluate the characteristics of the prepared nanoparticles. [ Methods] The PAMAM- STAT3- asODN- NP was Prepared with nanotechnology and antisense oligonucteotide .technology, and the resistance of nanoparticles to restriction enzyme and the stability of nanoparticles in different PH value were observed. [ Results] PAMAM- STAT3- asODN- NPs were not degradated in the range of pH2 to pill0, and had better resistance to restriction enzyme. [Conclusion] The nanoparticles has the characteristics of resisting restriction enzyme, acid-resistant and alkali-resistant. This experiment provides a basis for the utilization of the nanoparticles in gene therapy.

关 键 词：STAT3 反义寡核苷酸 纳米复合微粒 PH值 

分 类 号：R318.08[医药卫生—生物医学工程]