趋化因子受体CXCR4在神经胶质瘤中的表达及其临床意义  

EXPRESSION AND SIGNIFICANCE OF CHEMOKINE RECEPTOR CXCR4 IN GLIOMA

作　　者：丛璐璐[1] 胡红超[1] 焦保华[1] 

机构地区：[1]河北医科大学第二医院神经外科,河北石家庄050000

出　　处：《河北医科大学学报》2011年第7期770-773,F0002,共5页Journal of Hebei Medical University

摘　　要：目的观察CXCR4在正常脑组织及各级神经胶质瘤组织中的表达情况,探讨其与胶质瘤的侵袭性、病理级别及血管生成的关系。方法采用免疫组织化学方法测定5例正常脑组织及38例胶质瘤组织中CXCR4、CD34的表达,实时荧光定量PCR法测定CXCR4mRNA的相对表达量。结果 CXCR4在正常脑组织中阴性表达,胶质瘤组织中阳性表达率为63.16%,各病理级别组的阳性表达率及相对表达量差异均有统计学意义(P<0.05),且均随级别增加而升高;CD34单克隆抗体标记的微血管密度(microvessel density,MVD)在正常脑组织和脑胶质瘤组中的表达均值分别为3.76±0.48和35.25±9.75,在各级别组中的表达均值差异均有统计学意义(P<0.01)并随级别增加而升高;MVD在CXCR4表达阴性和阳性的胶质瘤组织中表达均值分别为26.37±5.11和40.43±7.88,差异有统计学意义(P<0.01)。结论 CXCR4与胶质瘤的侵袭性及恶性程度有关并可能参与胶质瘤的新生血管生成;MVD可作为判定肿瘤恶性程度和预后的重要指标。Objective To observe the expression of CXCR4 in normal brain tissue and all grade gliomas , analyze the internal relations between the expression of chemokines receptors CXCR4 and invasiveness of glioma, pathological grade and angiogenesis. Methods The expression of CXCR4 protein and CD34 were examined by immunohistochemistry in 5 normal brain tissue specimens and 38 glioma tissue specimens, the relative expression of chemokines receptors CXCR4 was detected in glioma tissue specimen by quantitative real - time PCR. Results The chemokines receptors CXCR4 was negatively expressed in normal brain tissues,the total positive expression rates in tumor group was 63. 16%, the positive expression rates and the relative expression of CXCR4 has significant difference between different grade gliomas （ P 〈 0.05 ）, and increased with the pathological grade of glioma; the CD34 monoclonal antibody- labeled- microvessel density （MVD） in normal brain tissue group and tumor group respectively were 3.76 ± 0.48 and 35.25 ± 9.75, the value had significant difference between different grade gliomas （P 〈 0.01 ）, and increased with the pathological grade of glioma; The expression average of MVD respectively were 26.37 ± 5.11 and 40.43 ± 7.88 in negative expression and positive expression of CXCR4 in glioma tissues, the data had very significant difference （ P 〈 0.01 ）. Conclusion CXCR4 is related to the invasiveness and the malignancy of glioma, and might take part in the angiogenesis of glioma; MVD can be used as an important indicator of determining malignancy and prognosis of tumor.

关 键 词：受体 趋化因子 神经胶质瘤 免疫组织化学 

分 类 号：R739.41[医药卫生—肿瘤]