胃癌组织中KiSS-1 mRNA及蛋白的表达  

Expression of KiSS-1 protein and mRNA in gastric cancer tissue

作　　者：崔广飞[1] 高凌[1] 

机构地区：[1]商丘市第一人民医院普外一科,河南商丘476100

出　　处：《中国医学工程》2011年第6期20-21,25,共3页China Medical Engineering

摘　　要：目的探讨胃癌组织中KiSS-1 mRNA及蛋白的表达情况。方法分别采用原位杂交方法和免疫组织化学SP法对40例胃癌组织、22例癌旁不典型增生组织及40例正常胃黏膜组织进行KiSS-1 mRNA和蛋白的检测。结果胃癌组织、癌旁不典型增生组织及正常胃黏膜组织中KiSS-1 mRNA的阳性表达率分别为57.5%(23/40)、77.3%(17/22)、97.5%(39/40),依次升高(X2=18.323,P<0.01);KiSS-1蛋白的阳性表达率分别为52.5%(21/40)、81.8%(18/22)、95.0%(38/40),依次增高(X2=20.131,P<0.01);胃癌组织中KiSS-1 mRNA及蛋白表达与胃癌分化程度无关(P>0.05),与浸润深度、淋巴结转移有关(P<0.05);胃癌组织中KiSS-1 mRNA与蛋白的表达呈正相关(rs=0.702,P<0.05)。结论 KiSS-1表达缺失与胃癌的发生、转移有关;KiSS-1有望成为胃癌早期诊断和判断预后的辅助指标。【Objective】 To investigate the expression of KiSS-1 mRNA and protein in gastric cancer tissue.【Methods】 The expression of KiSS-1 mRNA and protein in 40 cases of gastric cancer,22 cases of adjacent atypical hyperplasia epithelium and 40 cases of normal gastric mucosa tissues were detected by in situ hybridization and SP immunohistochemistry method,respectively.【Results】 The expression rates of KiSS-1 mRNA in gastric cancer tissue,adjacent atypical hyperplasia epithelium and normal gastric mucosa tissue,were 57.5%（23/40）,77.3%（17/22）,97.5%（39/40）,respectively,and increased by turns（X2= 18.323,P0.01）.The expression rates of KiSS-1 protein in carcinoma,adjacent atypical hyperplasia epithelium and normal gastric mucosatissue,were52.5%（21/40）,81.8%（18/22）,95.0%（38/40）,respectively,and increased by turns（X2=20.131,P0.01）.The mRNA and protein expression of KiSS-1 in gastric cancer tissue were correlated with depth of invasion and lymphatic metastasis（P0.05）,but not with in differentiation（P0.05）.There was positive correlation between the protein and mRNA expression of KiSS-1 in gastric cancer tissue（rs= 0.702,P0.05）.【Conclusion】 The loss expression of KiSS-1 is closely correlated to the formation and metastasis of gastric cancer,and KiSS-1 maybe a new assistant index for early diagnosis and prognosis for gastric cancer.

关 键 词：胃癌 KISS-1 原位杂交方法 免疫组织化学SP法 

分 类 号：R735.2[医药卫生—肿瘤]