实时荧光定量PCR技术在转基因食品检测领域中的应用  

Development of Real-Time Fluorescent Quantitative PCR in the Field of Genetically Modified Food Detection

作　　者：吴永彬[1] 肖维威[1] 马文丽[1] 

机构地区：[1]南方医科大学基因工程研究所,广东广州510515

出　　处：《生物技术通讯》2011年第4期575-579,共5页Letters in Biotechnology

基　　金：国家公益性行业科研专项(200910265);广东省科技计划(2008B080701027)

摘　　要：随着基因工程技术在农业生产中应用的深入,越来越多具有改良特征的转基因植物在全球范围内得到广泛种植,随之而来的转基因食品也迅猛发展,转基因产品大规模商业化引起了对安全性问题的担忧。为保证转基因产品标签制度的顺利实施,建立快速、准确、高通量的定量检测方法十分必要。我们综述了国内外转基因食品检测技术的研究进展,重点阐述了实时荧光定量PCR技术在转基因食品检测领域中的应用,并展望了通过构建质粒标准分子的方法来实现对更多转基因植物品系的定量检测。With wide application of genetic engineering technology in agricultural production,more and more improved characteristics of transgenic plants have been widely planted on a global scale,followed by genetically modified（GM） foods have to be developed rapidly.Because of security issues caused by large-scale commercialization of GM products,and ensuring GMO labeling system implement smoothly,a rapid,accurate,high-throughput quantitative assay is necessary.In this paper we reviewed the development of GM foods detection at home and abroad research progress,and focused on the application of real-time fluorescent quantitative PCR in the field of GM foods detection,and in its application prospects,constructing standard reference molecules were proposed to achieve more transgenic plant lines for quantitative detection.

关 键 词：转基因食品 实时荧光定量PCR 质粒标准分子 检测方法 

分 类 号：Q789[生物学—分子生物学]