内毒素诱导急性肺损伤大鼠血清对内皮细胞通透性的影响及乌司他丁的保护作用研究  

Effects of serum of rats with LPS-induced acute lung injury on endothelial cell permeability and the protective effects of ulinastatin

作　　者：薛新[1] 刘志勇[1] 

机构地区：[1]东南大学附属中大医院心胸外科,江苏南京210009

出　　处：《东南大学学报（医学版）》2011年第4期594-597,共4页Journal of Southeast University(Medical Science Edition)

摘　　要：目的:研究乌司他丁对大鼠急性肺损伤(ALI)的保护作用。方法:将15只SD大鼠分成3组:A组为健康对照组,B组为内毒素(LPS)诱导ALI组,C组为LPS诱导ALI应用乌司他丁治疗组。收集3组大鼠的血清分别作用于大鼠肺微血管内皮细胞(RPMVEC),观察血清刺激后RPMVEC通透性的改变及F-肌动蛋白的变化。结果:B组大鼠的血清使RPMVEC通透性明显增加,C组大鼠的血清明显减小RPMVEC的通透性。3组的通透系数与未加血清对照组通透系数的百分比依次为(7.31±0.27)%、(30.13±1.24)%、(18.94±0.59)%,组间比较差异均有统计学意义(P<0.05)。B组大鼠的血清可使RPMVEC F-肌动蛋白的荧光强度明显降低、F-肌动蛋白解聚,C组大鼠血清则明显减低F-肌动蛋白的解聚程度,3组间差异有统计学意义(P<0.05)。结论:LPS诱导ALI大鼠的血清可引起RPMVEC单层通透性增高与F-肌动蛋白解聚;乌司他丁可减轻ALI过程中炎症介质对内皮细胞骨架的影响,改善内皮细胞的通透性。Objective： To investigate the effects of serum of rats with LPS-induced acute lung injury on endothelial cell permeability and the protective effects of ulinastatin.Methods： Fifteen healthy male SD rats were divided into 3 groups,namely group A as the control group,group B with LPS-induced acute lung injury,group C with LPS-induced acute lung injury plus ulinastatin treatment.Rat pulmonary microvascular endothelial cell（RPMVEC） was isolated and culture from SD rat in vitro.The serum was collected from rats and added into RPMVEC.The effects of serum on monolayer permeability of RPMVEC were observed in transwell,and F-actin expression was evaluated by flow cytometry.Results： Serum of group B induced the increased permeability of RPMVEC monolayer and depolymerization of F-actin.Pretreatment with ulinastatin could lessen these changes.The percentage in change of permeability coefficient（△Pa%） after stimulation with the serum of rats in group A,B and C was（7.31±0.27）%,（30.13±1.24）%,（18.94±0.59）%,respectively,showing statistically significant differences（P0.05）.And the fluorescent numbers of F-actin were 1 344.26±12.78,654.63±48.28,321.32±32.14,showing statistically significant differences（P0.05）.Conclusion： The pro-inflammatory mediators in the serum of rats with LPS-induced acute lung injury increases RPMVEC permeability,and pretreatment with ulinastatin can lessen the hyperpermeability by inhibiting multiple pro-inflamatory mediators.

关 键 词：急性肺损伤 血清 大鼠肺微血管内皮细胞 单层细胞通透性 F-肌动蛋白 乌司他丁 

分 类 号：R-33[医药卫生] R965