Notch 3沉默对人急性T淋巴细胞白血病细胞增殖与凋亡的影响  

Effect of Notch 3 silencing on apoptosis and proliferation of acute T-cell lymphoblastic leukemia cells

作　　者：项捷[1] 欧阳永日[1] 崔颖[1] 林芳[1] 任继鸿[1] 龙敏[1] 陈曦[1] 卫军霞[1] 张惠中[1] 

机构地区：[1]第四军医大学唐都医院中心实验室,陕西西安710038

出　　处：《现代肿瘤医学》2011年第8期1482-1487,共6页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:30772181)

摘　　要：目的:探讨Notch 3受体特异性沉默对急性T淋巴细胞白血病的影响。方法:运用survivin启动子介导的RNA干涉方法特异性地封闭急性T淋巴细胞白血病细胞SupT1中Notch 3基因的表达。结果:survivin启动子介导的RNA干涉系统能特异而高效封闭肿瘤细胞内源Notch 3基因的表达,Notch 3基因表达的下调能够使肿瘤细胞的增殖活性降低,凋亡明显增加。结论:Notch 3基因沉默可有效抑制白血病细胞的增殖,并促进其凋亡,为急性T淋巴细胞白血病相关基因功能研究及靶向性治疗探索了一种新策略。Objective：To explore the effects of silencing of Notch 3 on the development of T-cell acute lymphoblastic leukemia.Methods： We investigated the effect of Notch 3 silencing on SupT1 cells using short hairpin RNA（shRNA） driven by survivin promoter.Results： shRNA targeting Notch 3 could down-regulate the expression of Notch 3 effectively.Down-regulating expression of Notch 3 correlated with a significant apoptosis and a proliferation inhibition.Conclusion： Notch silencing by shRNA driven by surviving promoter inhibits the proliferation and promotes the apoptosis of SupT1.This study provides an alternate therapy strategy against Notch signaling pathway.

关 键 词：NOTCH3 SURVIVIN启动子 急性T淋巴细胞白血病 

分 类 号：R730.54[医药卫生—肿瘤] R733.71[医药卫生—临床医学]