子宫内膜癌耐药细胞B-MD-C1(ADR^(+/+))与非耐药细胞B-MD-C1的蛋白质差异研究  

Identification of differentially expressed proteins in B-MD-C1(ADR^(+/+)) and B-MD-C1 by proteomics

作　　者：张超[1] 武立华[1] 艾军[1] 单保恩[1] 

机构地区：[1]河北医科大学第四医院科研中心,河北石家庄050011

出　　处：《癌变．畸变．突变》2011年第4期263-268,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：河北省普通高校强势特色学科资助项目(冀教高[2005]52);河北省自然科学基金资助项目(C2009001195)

摘　　要：目的:比较子宫内膜癌耐药细胞B-MD-C1(ADR^(+/+))与非耐药细胞B-MD-C1的蛋白质表达谱,识别和鉴定差异表达蛋白,分析差异蛋白与其耐药的关系。方法:采用双向凝胶电泳及基质辅助激光解吸电离-飞行时间质谱法对B-MD-C1(ADR^(+/+))与BMD-C1细胞进行蛋白质分离和表达谱研究,利用生物信息学方法鉴定差异蛋白质。结果:B-MD-C1细胞与B-MD-C1(ADR^(+/+))相比,蛋白质表达差异>2倍的蛋白质点有35个,其中18个蛋白点表达上调,17个蛋白点表达下调。应用Mascot软件查询Swiss-prot数据库初步鉴定差异表达的蛋白质点24个,这些蛋白质可能是B-MD-C1细胞产生耐药的相关蛋白,包括细胞骨架蛋白、代谢酶类、细胞周期相关蛋白、信号转导和凋亡调控相关蛋白、蛋白合成和参与糖酵解的蛋白等。结论:热休克蛋白、β-微管蛋白、丙酮酸激酶同工酶、核糖体蛋白等是B-MD-C1(ADR^(+/+))与B-MD-C1细胞间的差异蛋白,这些蛋白多与B-MD-C1(ADR^(+/+))细胞耐药密切相关。OBJECTIVE：Multidrug resistance（MDR） is a major obstacle for effective treatment of tumors. Previous studies of MDR tended to concentrate in a single or a few related genes and their protein products.We focused on the total protein with the method of proteomics.The aim of this study was to investigate differently expressed protein in BMD -C1 and B-MD-C 1（ADR^（＋/＋）） cells by proteomic analysis.METHODS：The holoproteins of human carcinoma of endometrium cell lines B-MD-C1 and B-MD-C1（ADR^（＋/＋）） were measured by two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS）.RESULTS：Thirty-five differential proteins were analyzed by peptide mass fingerprinting.The differentially expressed proteins could be divided into six groups based on their functions：molecular chaperones,cystoskeleton proteins（actin-related protein 3,lamin-B1 and tubulin beta chain）,metabolic enzymes（78 kDa glucose-regulated protein,retinal dehydrogenase）,proteins associated with cell cycle（eukaryotic initiation factor 4A-Ⅲ）,proteins associated with cell proliferation,differentiation and apoptosis（endoplasmic reticulum protein,endoplasmic reticulum protein）,and proteins associated with carbohydrate metabolism（60S acidic ribosomal protein,alpha-enolase）.CONCLUSION：These differentially expressed proteins provided some clues to the mechanism of tumor cell resistant to cisplatin,providing the basis of searching for potential target of carcinoma.

关 键 词：子宫内膜癌 蛋白质组学 多药耐药性 质谱分析 

分 类 号：R730.2[医药卫生—肿瘤]