补体C4b及甲状腺素转运蛋白在增生性玻璃体视网膜病变中的表达变化及意义  

Differential expression and significance of complement C4b and transthyretin in proliferative vitreoretinopathy

作　　者：陈国海[1] 李涛[1] 郑钦象[1] 侯江平[1] 唐仕波[2] 李文生[1] 

机构地区：[1]温州医学院附属眼视光医院,325027 [2]中山大学中山眼科中心眼科学国家重点实验室

出　　处：《中华眼科杂志》2011年第8期726-731,共6页Chinese Journal of Ophthalmology

基　　金：浙江省自然科学基金，国家眼科学重点实验室开放课题基金

摘　　要：目的探讨补体C4b及甲状腺素转运蛋白（YrR）在增生性玻璃体视网膜病变（PVR）中的表达变化及意义。方法对照实验研究。提取5例PVR患者玻璃体标本，以10例眼球捐献者的玻璃体标本作为正常对照组，进行双向电泳分析。应用Imagemaster软件分析凝胶图谱获得的差异性蛋白质点，再经质谱分析技术鉴定出差异性蛋白质。兔眼玻璃体腔内注射视网膜色素上皮细胞，制造兔PVR模型，采用酶联免疫吸附测定（ELISA）法检测玻璃体中差异性蛋白质的浓度，以进一步验证PVR患者的蛋白质组学结果。PVR患者组与正常对照组玻璃体中差异性蛋白质浓度比较采用配对样本t检验。结果双向电泳凝胶图谱显示PVR患者组与正常对照组玻璃体中有79个差异表达蛋白质点，对其中9个表达上调的差异性蛋白质点进行质谱鉴定，分别为补体CAb、TTR及7个血清蛋白。经ELISA法检测，正常对照组玻璃体中补体C4b和rrrR浓度分别为（20．18±1．97）mg／L和（88．58±8．84）mg／L，PVR患者组玻璃体中补体和1TrR浓度分别为（38．1±5．79）mg／L和（112．57±6．89）mg／L；PVR患者组玻璃体中的补体CAb和TTR浓度明显高于正常对照组，差异有统计学意义（CAb：t=11．54，TTR：t=9．24；P〈0．05）。结论PVR患者组与正常对照组玻璃体中补体CAb和1TTR表达存在差异。补体C4b和TTR的表达上调可能与PVR的发病机制有关。（中华胺科杂志，2011．47：726．731）Objective To investigate the differential expression of complement C4b and transthyretin in proliferative vitreorefinopathy （PVR）. Methods It was a controlled experimental study. Human vitreous samples of 5 patients with PVR were analyzed by using two-dimensional gel electrophoresis and mass spectrometry, and the results were compared with those from normal control vitreous obtained from donor eyes. An in vivo model of PVR was created by intravitreous injection of cultured rabbit retinal pigment epithelial （RPE） cells. The vitreous of PVR models were analyzed by enzyme linked immunosorbent assay （ELISA） to confirm the proteomic results from the PVR patients. Results Seventy nine various proteins were expressed differently between PVR and normal vitreous, among which nine up-regulated proteins including complement C4b, transthyretin （TTR）, and 7 albumins were identified by mass spectrometry. The up-regulation of complement CAb and TTR in PVR patients was also confirmed by ELISA. The concentration of complement C4b and TTR in normal vitreous were （ 20. 18 ± 1.97 ） mg/L and （ 88.58 ± 8.84 ） mg/L respectively, in PVR patients were （ 38.1 ± 5.79 ） mg/L and （ 112.57 ± 6. 89 ） mg/L repectively, difference significantly between these two groups （ CAb： t = 11. 54, TFR： t = 9. 24; P 〈 0. 05 ）. Conclusions Differences of complement CAb and TFR expression were observed between PVR and normal vitreous. These results have lead to the assumption that there is a connection between elevated concentrations of both complement C4b and TTR and the pathogenesis of PVR and further studies on the functions of these proteins are required. （Chin J Opthalmol , 2011,47：726-731 ）

关 键 词：玻璃体视网膜病 增生性 补体C4b 甲状腺素转运蛋白 蛋白质组学 电泳 凝胶 双向 质谱分析法 

分 类 号：R774.1[医药卫生—眼科]