帕金森病大鼠脑局部葡萄糖代谢及多巴胺2型受体表达的microPET研究  

A study of microPET for assessing regional cerebral glucose metabolism and the expression of dopamine receptor type 2 in a rat model of Parkinson＇s disease

作　　者：李天瑯[1] 徐建飞[2] 王菁[3] 张宏[3] 毛善英[4] 闻树群[4] 周燕[5] 

机构地区：[1]浙江大学医学院附属第二医院干部保健科,杭州310009 [2]浙江大学医学院附属第二医院急救医学研究所,杭州310009 [3]浙江大学医学院附属第二医院PET中心,杭州310009 [4]浙江大学医学院附属第二医院神经内科,杭州310009 [5]浙江大学医学院附属第二医院病理科,杭州310009

出　　处：《中华医学杂志》2011年第29期2068-2072,共5页National Medical Journal of China

基　　金：国家自然科学基金青年基金（30700883）;浙江省医药卫生科学研究基金（2006A065）

摘　　要：目的应用18F-FDG和11C-NMSPmicroPET评估大鼠帕金森病（PD）模型脑局部葡萄糖代谢及多巴胺2型受体（DRD2）表达的差别及意义。方法采用6-羟多巴胺制备偏侧PD大鼠模型，2周后应用18F-FDG和11C-NMSP microPET扫描，比较PD模型组大鼠和对照组大鼠的脑局部葡萄糖代谢及DRD2表达，用免疫组化染色比较两组的酪氨酸羟化酶表达。结果PD模型大鼠纹状体、海马、感觉运动皮质葡萄糖代谢率分别为88．2％±2．2％，94．5％±4．5％，96．2％±5．8％，显著低于对照组相应脑区92．7％±2．8％（P〈0．01），98．9％±3．9％（P〈0．01），102．8％±2．1％（P〈0．01）；DRD2表达在PD模型的右侧纹状体为112．9％±9．O％，明显高于对照组102．3％±1．4％（P〈0．01）。结论PD模型大鼠损毁侧纹状体、海马、感觉运动皮质葡萄糖代谢均降低；而损毁侧纹状体DRD，表达明显升高。18F-FDG和11C—NMSP microPET能有效评估PD模型脑局部葡萄糖代谢及DRD2表达水平，可能成为早期诊断PD的分子影像学工具。Objective To employ 18F-fluoro-2-deoxyglucose （18F-FDG） and （3-N-[11C] methylspiperone） 11C -NMSP microPET to assess the changes of regional cerebral glucose metabolism and the expression of dopamine receptor type 2 （ DRD2 ） in a rat model of Parkinson＇s disease （PD）. Methods A hemiparkinsonian model was established in rats by unilateral pretreatment with 6-hydroxydopamine （6- OHDA）. At 2 weeks after 6-OHDA insult, 18F-FDG and 11C-NMSP microPET scan were performed to compare the differences of regional cerebral glucose metabolism and the expression of DRD2 between the PD and control groups respectively. The immunohistochemical staining was used to detect the expression of tyrosine hydroxylase in two groups. Results In the PD model, the glucose metabolism rates were 88. 2% ± 2. 2%, 94. 5% ±4. 5% and 96. 2% ±5.8% respectively, in right striatum, hippocampus and sensorimotor cortex. And they were significantly lower than those in the control group [ 92. 7% ± 2. 8% （ P 〈 0. 01 ）, 98.9% ± 3.9% （P 〈 0. 01 ） ＆ 102. 8% ±2. 1% （ P 〈 0. 01 ） ]. The expression of DRD2 in right striatum was significantly higher in the PD group than that in the control group （ 112. 9% ± 9. 0% vs 102. 3% ± 1.4% , P 〈 0. 01 ）. Conchlsion In the PD rats, glucose metabolism decreases in injured side striatum, hippocampus and sensorimotor cortex while and the expression of DRD： increases in injured side striatum. 18F-FDG and 11C-NMSP microPET can effectively assess the regional cerebral glucose metabolism and the expression of DRD2 in PD. They may serve as effective molecular imaging tools for an early diagnosis of PD.

关 键 词：帕金森病 正电子发射断层显像 葡萄糖代谢 多巴胺2型受体 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]