粘虫颗粒体病毒与苏云金杆菌联合作用对甜菜夜蛾的影响  

Impact of Bacillus thuringiensis and Pseudaletia unipuncta Granulovirus-Ps on Spodoptera exigua

作　　者：刘琴[1] 徐健[1] 祁建杭[1] 施建德[1] 李传明[1] 马谈斌[1] 

机构地区：[1]江苏里下河地区农业科学研究所,江苏扬州225007

出　　处：《生物安全学报》2009年第2期124-129,共6页Journal of biosafety

基　　金：江苏省自然科学基金(CX07B-2009);江苏省资源开发局项目(2009KJ-20)

摘　　要：以甜菜夜蛾为试虫,测定了粘虫颗粒体病毒(PuGV-Ps)对苏云金杆菌(Bt)毒力的增效作用。结果表明PuGV对甜菜夜蛾没有致毒作用,但Bt中加入PuGV后可以提高Bt对甜菜夜蛾的毒力,甜菜夜蛾致死中量LC_(50)由Bt单剂的1.094 mg/mL下降到0.862 mg/mL,共毒系数达127。亚致死剂量Bt处理甜菜夜蛾影响了幼虫的生长发育,表现为幼虫生长量相对减少、蛹重下降、化蛹率降低和化蛹历期延长,添加了PuGV-Ps后进一步增强了Bt对甜菜夜蛾的生长发育的抑制作用。甜菜夜蛾中肠蛋白酶活性测定结果表明,PuGV-Ps对甜菜夜蛾中肠酶活性具有抑制作用;昆虫同时取食PuGV-Ps和Bt后,中肠酶液总蛋白酶活力都有所下降,在中肠酶液最适pH范围内蛋白酶活力抑制作用最明显。Enhancement of toxicity of Bacillus thuringiensis （Bt） with Pseudaletia unipuncta Granulovirus-Ps （PuGV-Ps） was dem- onstrated by bio-assays employing larvae of S. exigua. Combinations of Bt and PuGV-Ps were synergistic and enhanced toxicity of Bt while PuGV-Ps represented no effect to the larve. The co-toxicity coefficients （CTC） of Bt combined with PuGV-Ps was 127. Sub - deadly dosage of Bt influenced the larval growth of S. exigua, with less weight of larvae and pupae, delay of pupating and lower ratio of pupation. PuGV-Ps also enhanced the effect of Bt on the development of larvae. The proteolytic activity of midgut juice from Spodotera exigua was influenced by PuGV-Ps. When larvae of S. exigua swallowed the feed contain some Bt and PuGV-Ps, the total proteolysis was reduced for some degree by PuGV-Ps under the suited pH value of midgut juice.

关 键 词：粘虫颗粒体病毒 苏云金杆菌 甜菜夜蛾 蛋白酶活性 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]