二十碳五烯酸抑制肺腺癌A-549细胞株增殖  

The Effect of Inhibiting Hyperplasia of Eicosapentaenoic Acid on A-549 Human Lung Cancer Cell Line

作　　者：马刘江[1] 安京华[1] 李星[1] 石俊[1] 

机构地区：[1]延边大学附属医院,吉林延吉133000

出　　处：《中国现代医生》2011年第23期7-9,共3页China Modern Doctor

基　　金：国家自然科学基金资助项目(30560152);吉林省教育厅自然科学重点研究计划项目

摘　　要：目的在体外细胞实验中,观察二十碳五烯酸(EPA)联合顺铂(DDP)对肺腺癌A-549细胞株的抗增殖和诱导凋亡作用。方法选用A-549细胞株进行体外培养,用MTT比色法测定EPA对A-549细胞株的抗增殖作用。应用倒置显微镜、光学显微镜观察细胞凋亡形态学变化。应用TUNEL染色法检测细胞凋亡指数(AI)。结果实验结果表明,EPA在(60～120)μg/mL浓度范围内,于24、48、72、96h四个时间段对肺腺癌A-549细胞株均有抑制生长作用,且EPA与顺铂有协同抗癌作用,并表现出浓度、时间依赖性关系。当EPA浓度为60、80、100、120μg/mL时,细胞凋亡指数(AI)依次升高,分别为(81.52±1.96)%、(83.74±2.21)%、(85.30±2.38)%、(86.26±2.44)%,不同浓度组相比差异有统计学意义(P<0.05)。结论体外实验中,EPA对A-549细胞生长有抑制作用,呈明显的量效和时效关系,且与顺铂有协同作用。Objective To observe the effects of anti-proliferation and inducing apoptosis on A-549 cell line induced by eicosapentaenoic acid （ EPA ） and its synergistic effect with cisplatin （ DDP ） in vitro. Methods A-549 cell line was cultured in vitro. MTT assay was used to determine the growth inhibitory effect of EPA on A-549 cells. Morphological changes of the apoptosis of A-549 cells were observed by using inverted microscope. TUNEL was used to detect cell cycle distribution and apoptotic rate of A-549 cells. Results The results of experiments showed that when A-549 cells were exposed to EPA for 24h, 48h, 72h and 96h respectively at final concentration of （ 60-120 ）μg/mL, the growth of cells was inhibited and showed in dose and time dependent manner. EPA combined with DDP showed significant synergistic anti-tumor effect on A-549 cells. When the concentration of EPA altered from 60 μ g/mL to 120μ g/mL, the apoptotic index （ AI ） of A-549 cells elevated accordingly by the method of terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling （ TUNEL ）, the apoptotic indexes were （ 81.52 ± 1.96 ） %, （ 83.74 ± 2.21 ） %, （ 85.30 ± 2.38 ） %, （ 86.26 ± 2.44 ） % respectively and showed statistical significance compared between different concentration （ P 〈 0.05 ）. Conclusion EPA inhibits growth of A-549 cell line in vitro in dose and time dependent manner; EPA combined with DDP shows significant synergistic anti-tumor effect on A-549 cell line.

关 键 词：二十碳五烯酸 增殖 肺腺癌 A-549细胞株 

分 类 号：R734.2[医药卫生—肿瘤]