食管鳞状细胞癌患者血清T辅助细胞类细胞因子的表达及其临床意义  

Expression of Th type cytokines in patients with esophageal squamous cell carcinoma and the clinical significance

作　　者：陈宇[1] 方建禅[1] 李永良[1] 

机构地区：[1]莆田学院附属医院肿瘤科,351100

出　　处：《中国综合临床》2011年第8期788-790,共3页Clinical Medicine of China

基　　金：莆田市科技计划项目（2007S10）

摘　　要：目的探讨食管癌患者血清T辅助细胞（Th1、Th2）类细胞因子的表达及其临床意义。方法采用双抗体酶联免疫吸附法（ELISA）检测45例食管鳞状细胞癌患者及30名健康体检者外周血Th1[干扰素（INF）-γ、白细胞介素（IL）-2]、Th2类（IL-4）细胞因子的含量。结果食管癌患者Th1类细胞因子INF-γ、IL-2表达含量较健康对照组降低[（11．51±2．15）、（16．31±2．27）ng／L，t=18．24，P〈0．01）；（10．20±2．10）、（16．92±2．41）ng／L，t=15．31，P〈0．05]；Th2类细胞因子（IL-4）较健康对照组升高[（5．30±0．11）、（2．23±0．29）ng／L，t=5．79，P〈0．05]，且其表达趋势与肿瘤的TNM分期有关。结论食管癌患者体内存在Th1／Th2漂移现象。该现象可能与肿瘤细胞发生免疫逃逸有关。Objective To study the expression of Thl and Th2 types cytokines in patients with esophageal squamous cell carcinoma and the clinical significance. Methods The expressions of Thl （ INF-γ.IL-2） and Th2 （IL-4） types cytokines in cultured peripheral blood mononuclear cells （PBMC） from 30 healthy controls and 45 esophageal squamous cell carcinoma patients were determined by Enzyme-linked immunosorbent assay （ELISA）. Results Compared with the control group, the expressions of Thl type cytokines, IL-2 and INF-γ were significantly lower in the cultured PBMCs from the cancer patients （TNF-γ, = 18.24 ,tIL-2 = 15.31 ,Ps 〈 0. 01 ） , while the expression of Th2 type cytokines, IL-4 was significantly higher in the cultured PBMCs from the cancer patients （ tIL-4 = 5.79, P 〈 0. 05 ）. The expression changes of the cytokines showed a correlation with TNM （tumou-node-metastasis） stage of the tumor. Conclusion The Th1/Th2 shift existed in patients with esophageal squamous cell carcinoma and it might be one of the underline mechanisms of tumor immune escape.

关 键 词：食管癌 Th类细胞因子 干扰素-Γ 白细胞介素 

分 类 号：R735.1[医药卫生—肿瘤]