甲状腺乳头状癌患者血液p53基因表达及与淋巴结转移、临床分期的关系  

Expression of serum p53 gene and its correlation with lymphatic metastasis and clinical stages in patients with thyroid papillary carcinoma

作　　者：尹凤媛[1] 焦淑贤 迟晓云 

机构地区：[1]青岛市市立医院,山东青岛266011 [2]青岛市红十字会中心血站

出　　处：《山东医药》2011年第27期25-26,共2页Shandong Medical Journal

基　　金：青岛市市南区科技发展计划资助项目(2009-5-21-GG)

摘　　要：目的探讨p53基因突变在甲状腺乳头状癌(PTC)发生、发展中的作用。方法选择118例PTC患者(PTC组)、38例淋巴结转移甲状腺癌患者(转移组)及122例健康体检者(对照组),均抽取空腹静脉血,采用PCR-单链构象多态方法检测p53基因(第5-8外显因子)表达情况。结果 PTC组和转移组血液p53基因表达阳性率均显著高于对照组,其中转移组显著高于PTC组,PTC组Ⅲ～Ⅳ期者显著高于Ⅰ～Ⅱ期者(P均<0.05)。结论 p53基因突变可促进PTC的发生和转移;可为PTC的诊治及预后判定提供依据。Objective To explore the role of p53 gene mutation in the occurrence and development of thyroid papillary earcinoma（PTC）. Methods One hundred and eighteen patients with PT（PTC group）, 38 cases of thyroid carcinoma with lymphatic metastasis（metastasis group） and 122 healthy controls（ control group） were enrolled in, the 5-8 exons of p53 gene of the three groups was monitored using polymerase chain reaction-single strand conformation polymorphism（ PCR-SS- CP）. Results p53 mutation rates in PTC group and metastasis group were both higher significantly than that in control group, especially in the metastasis group, those of patient in Ⅲ-ⅣIV stage were significantly higher than with that in Ⅰ --Ⅱ （ all P 〈 0.05 ）. Conclusions p53 gone mutation may improve the occurrence and metastasis of PTC; this can provide basis for the diagnosis and treatment, decision of prognosis of PTC.

关 键 词：甲状腺乳头状癌 P53基因 聚合酶链式反应-单链构象多态 

分 类 号：R736.1[医药卫生—肿瘤]