灵五颗粒保肝退黄降酶作用研究  

Study on the Liver Protection,Jaundice Receding and Liver Enzyme Decreasing Effect of Lingwu Granula

作　　者：徐雪钰[1,2] 刘军[2] 于珊[2] 刘青川[2] 程丽娟[2] 黄正明[2] 

机构地区：[1]解放军第309医院药学部,北京100091 [2]解放军第302医院药剂科,北京100039

出　　处：《医药导报》2011年第8期994-997,共4页Herald of Medicine

基　　金：医院制剂基金项目(基金编号:201002-03)

摘　　要：目的观察灵五颗粒对α-萘异硫氰酸酯(α-naphthylisothiocyanate,ANIT)致黄疸型肝损伤模型小鼠的保护作用及作用机制。方法小鼠随机分为正常组,模型组,灵五颗粒低、中、高剂量组(2.5,5.0,10 g.kg-1)和阳性对照组(茵栀黄颗粒2.34 g.kg-1)。正常组和模型组给予0.9%氯化钠溶液,其余各组连续灌胃给药7 d。除正常组外,其他各组末次给药1 h后灌服ANIT,105 mg.kg-1,制备小鼠肝损伤黄疸模型。48 h后取血、肝脏,分别测定血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、总胆红素(total bilirubin,T-BiL),肝脏组织中丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽(glutathione,GSH)等,并检查肝脏病理学。结果与正常组比较,模型组小鼠血清T-BiL、ALT、AST含量明显升高。灵五颗粒中、高剂量组小鼠血清T-BiL、ALT、AST含量均降低,并呈剂量依赖关系;与模型组相比,差异有统计学意义(P<0.01)。LW中、高剂量组也能显著降低受损肝组织中MDA含量,同时升高SOD、GSH活性。结论灵五颗粒对ANIT所致小鼠黄疸型肝损伤具有保护作用,退黄降酶效果明显,其作用机制可能与抗氧化作用有关。Objective To study the liver protective effects and mechanism of lingwu granula on liver injury in a mice model of jaundice induced by α-naphthylisothiocyauate （ANIT）. Methods Mice were randomly divided into six groups： normal group, ANIT model group, lingwu granula treatment groups （ 2. 5,5, 10 g · kg^-1 ） and yinzhihuang granula group （2.34 g · kg^-1 ）. After intragastric administration of certain medicines in each group for 7 days except normal and ANIT model groups which were treated with NS,mice were i. g. to induce jaundice-type liver injury model （ 105 mg·kg^-1 ） . The blood and liver tissue were collected to detect the serum contents of alanine aminotransferase（ALT） , aspartate aminotransferase（ AST）, total bilirubin（T-BiL） and the activities of malondialdehyde （ MDA ）, superoxide dismutase （ SOD ）, glutathione （ GSH ） in liver. The hepatic histopathology was detected. Results The contents of T-BiL, ALT, AST of ANIT model group were increased obviously compared with those in the normal group. However,lingwu granula significantly decreased the content of ALT, AST, T-BiL in serum in a good dose-depend manner compared with ANIT model group （P〈0.01）. In addition, lingwu granula at middle and high dose significantly lowered the activities of MDA （P〈0.01） and reinforced the activities of SOD （P〈0.01） and GSH （ P〈 0.05 and P〈0.01 ） in liver as well. Conclusion Lingwu granula protects liver injury induced by ANIT, brings down liver enzyme and recedes jaundice, which may be related to its antioxidation effect.

关 键 词：灵五颗粒 退黄作用 降酶作用 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]