预防使用米诺环素对胸段脊髓全横断大鼠运动功能恢复的影响  

EFFECTS OF MINOCYCLINE PRETREATMENT ON MOTOR FUNCTIONAL RECOVERY OF COMPLETE SPINAL CORD TRANSETION RATS

作　　者：陈萌[1] 赵淑敏[1] 马泉[1] 郑小影[1] 孔祥玉[1] 

机构地区：[1]承德医学院基础医学部,河北承德067000

出　　处：《承德医学院学报》2011年第3期240-243,共4页Journal of Chengde Medical University

摘　　要：目的:观察预防使用米诺环素对脊髓全横断大鼠运动功能的作用。方法:30只雄性Wistar大鼠随机分为3组,假手术组、脊髓横断模型组和米诺环素预处理组,每组10只大鼠。米诺环素预处理组大鼠术前腹腔注射米诺环素(90mg/kg)。各组大鼠于术后1、7、14、21d应用改良Tarlov评分和BBB评分方法对大鼠后肢行为功能进行评价。第22d将所有实验大鼠处死后取损伤段脊髓,行HE染色。结果:术后第1d,脊髓横断模型组和米诺环素预处理组大鼠Tarlov评分障碍率、BBS评分比较无明显差别(P>0.05);米诺环素预处理组术后第7d到第21dTarlov评分障碍率明显低于脊髓横断模型组,BBB评分明显高于脊髓横断模型组(P<0.01)。HE染色结果显示,脊髓横断模型组大鼠脊髓出现明显病理变化,米诺环素预处理组大鼠脊髓的病理变化明显减轻。结论:预防使用米诺环素可明显促进胸段脊髓横断大鼠运动功能的恢复。Objective：To observe the effects of minocycline pretreatment on motor function of complete spinal cord transection rats.Methods：30 male Wistar rats were randomly divided into sham operation group,spinal cord transection model group and minocycline pretreatment group,10 rats in each group. Rats in minocycline pretreatment group were intraperitoneally injected minocycline（90mg/kg） before operation. Tarlov＇s score and BBS score were used to evaluate the motor function of rats’ hindlimb;HE staining to observe the morphology of damaged spinal cord 22d after operation.Results：1d after injury,the Tarlov＇s score and BBS score of rats in minocycline pretreatment group had no obvious differences compared with rats in model group（P0.05） . 7d-21d after injury,the Tarlov＇s score of rats in minocycline pretreatment group was obviously lower than that of rats in model group,while BBS score obviously higher（P0.01） . HE staining showed that the spinal cord of model rats had significant pathological changes,while the pathological changes of spinal cord of rats in minocycline pretreatment group lightened significantly.Conclusions：Minocycline pretreatment can significantly promote motor function recovery of complete spinal cord transection rats.

关 键 词：米诺环素 脊髓全横断损伤 Tarlov评分 BBB评分 

分 类 号：R744.9[医药卫生—神经病学与精神病学]