复方倍他米松抑制兔耳瘢痕增生与氧自由基作用的初步研究  

Inhibitory Effect of Triamcinolone Acetonide on Hypertrophic Scar and Oxygen Free Radicals in Rabbits

作　　者：吴新民[1] 李霞[2] 周勤生[1] 马龙[3] 白静[1] 谭戈[1] 

机构地区：[1]新疆医科大学第五附属医院整形美容科,新疆乌鲁木齐830011 [2]新疆医科大学第五附属医院麻醉科,新疆乌鲁木齐830011 [3]新疆兵团医院烧伤整形科,新疆乌鲁木齐830011

出　　处：《中国现代医生》2011年第21期9-11,共3页China Modern Doctor

摘　　要：目的了解复方倍他米松局部注射对兔耳增生性瘢痕组织中丙二醛含量的影响，并探讨其与氧自由基的关系。方法新西兰兔14只制作兔耳增生性瘢痕模型，正常兔耳皮肤组织标本8例；兔耳增生性瘢痕组织标本28例，随机分为复方倍他米松组（10例）、生理盐水组（10例）、空白对照组（8例）。术后6周予复方倍他米松（40mg／mL）分点注射于瘢痕样组织内，每处2～3点，总量0．3～0．4mL，每周1次，3次为一疗程。术后9周取材，计数成纤维细胞，并测量瘢痕的相对增生厚度，测定丙二醛含量变化。结果①大体形态学变化：复方倍他米松部治疗3周后，瘢痕颜色接近兔耳的正常肤色，略高出皮面，表面工整，触之质软。②组织学变化：与其他三组比较，复方倍他米松组胶原纤维多为平行排列，数量减少。③成纤维细胞密度与瘢痕增生指数变化：与其他三组比较，复方倍他米松组成纤维细胞密度增高（P〈O．05），④丙二醛含量变化：与其他三组比较，复方倍他米松组丙二醛含量明显增高（P〈0．05）；结论复方倍他米松局部注射兔耳创面引起兔耳增生性瘢痕组织中氧自由基水平升高。Objective To investigate the inhibitory effect of triamcinolone acetonide on hypertrophic scar and oxygen free radicals in rabbits. Methods Eighteen New Zealand rabbits were used. 14 rabbits were selected randomly to construct animal models of hypertrophic scar on the ears. Another 4 rabbits （ 8 ears ）were used as controls. 6 weeks after operation, the hypertrophic scar on the ears were randomly divided to receive intra-lesion injection of TA （ n=10 ）, or normal saline （n=10）, or nothing（ n=8, sham group ） .9 weeks after operation, scar specimens were taken for scar thickness measurement, fibroblast counting under microscopy and MDA content detection by spectrophotometric method. Results （1） 3 weeks after TA treatment, the scar became very thin and soft with a similar color to normal skin and a smooth surface; （2） Histologic study showed the collagen fibers in TA group were reduced markedly and arranged parallelly; （3） Compared with normal skin, the fibroblast density-in,sham and saline groups increased significantly （P 〈 0.05 ）, but the scar hypertrophic index was decreased dramatically in TA group （ P 〈 0.05 ） ; （4） The MDA content was highest in TA group （P 〈 0.05 ）, followed by that in sham and saline groups （P 〈 0.05 ）, It was lowest in normal control group （P 〈 0.05 ）. Conclusion The oxygen free radicals in the hypertrophic scar can be further increased by local injection of TA.

关 键 词：增生性瘢痕 复方倍他米松 氧自由基 细胞凋亡 成纤维细胞 

分 类 号：R782.2[医药卫生—口腔医学]