围冷冻手术期施行免疫疗法的抗肿瘤作用  

Antitumor effect of peri cryosurgical immunotherapy

作　　者：张保宁[1] 张叔人[1] 曲平[1] 周春霞[1] 袁兴华[1] 杨伟志[1] 王国佩[2] 李景敏[2] 关秋平[2] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院,北京100021 [2]北京民航总医院

出　　处：《中华肿瘤杂志》1999年第6期427-429,共3页Chinese Journal of Oncology

基　　金：中国医学科学院中国协和医科大学院校基金资助

摘　　要：目的　通过动物实验总结冷冻外科联合生物免疫治疗恶性肿瘤的经验,为临床应用提供依据。方法　将接种黑色素瘤B16 的荷瘤小鼠C57BL/6 随机分为冷冻免疫治疗组( 实验组) 、冷冻对照组、免疫对照组、盐水对照组。实验组在围冷冻手术期,去除免疫抑制,配合生物免疫治疗,强化抗肿瘤免疫作用;其他3 个对照组分别进行冷冻治疗、免疫治疗、注射生理盐水。治疗后比较各组疗效。结果　肿瘤接种后第35 天,实验组、冷冻对照组、免疫对照组、盐水对照组肿瘤体积分别为0 .4 ,1 .1 ,1 .9 ,3 .9 cm3 。若按90 天计算生存期,实验组与冷冻对照组、免疫对照组、盐水对照组的生存期分别为71 ,62 ,53 ,46 天。免疫对照组和盐水对照组无一例治愈;冷冻对照组的7 只小鼠中有1 只治愈;实验组的6 只小鼠中有3 只治愈。治愈小鼠3 个月后人为处死,原肿瘤部位组织切片经光镜、电镜检查均未见肿瘤细胞,其疗效明显优于其他各对照组( P< 0 .05) 。多次重复上述实验结果一致。结论　单纯冷冻或单纯免疫治疗非免疫原性肿瘤能减缓肿瘤的发展,但治愈率极低。若两种治疗方法联合应用,发挥优势互补,将明显提高疗效。Objective To evaluate the anti tumor effect of combined cryosurgery and immunotherapy in mice. Methods C 57 BL/6 mice bearing B 16 melanoma were randomly divided into 3 groups: (1) peri cryosurgical immunotherapy group, (2) cryosurgery group, (3) immunotherapy group and (4) saline group. Results The tumor size on day 35 was 0.4 cm 3 in group 1, 1.1 in group 2, 1.9 in group 3, and 3.9 in group 4 of mice. The survival period was 71, 62, 53 and 46 days, respectively. None in group 3 and 4 was cured, while 14% of mice in group 2 and 50% in group 1 were tumor tree. In tumor free mice, no tumor cell in the primary tumor sites was found by light and electron microscopy 3 months later.Conclusion The combined use of cryosurgery and immunotherapy is curative in 50% of mice bearing established B 16 melanoma.

关 键 词：冷冻外科 肿瘤 围手术期 免疫疗法 

分 类 号：R730.51[医药卫生—肿瘤]