乳腺癌腋窝淋巴结转移血管生成的免疫组化研究  

Im munohistochemical study of angiogenesis of axillary lymph node metastasisin human breast carcinoma

作　　者：武利存[1] 张维东[1] 刘惠萍[2] 赵斌[3] 孙公甲[1] 

机构地区：[1]山东省医学科学院基础医学研究所,济南250062 [2]济南市中心医院病理科 [3]山东医科大学校医院

出　　处：《中华肿瘤杂志》1999年第6期434-435,共2页Chinese Journal of Oncology

基　　金：山东省科委资助

摘　　要：目的　研究乳腺癌腋窝淋巴结转移的血管生成情况。方法　采用内皮细胞ⅧFRAg 免疫组化染色技术,对37 例乳腺癌根治术或改良根治术切除的乳腺癌组织和121 枚腋窝转移淋巴结进行免疫组化染色。在100 倍视野下通过显微电视系统计数微血管密度( MVD) ,并用显微测量器测量转移灶的直径。结果　在121 个淋巴结中找到13 处微转移灶,其平均直径为(210 ±37) μm ,无血管生成。腋窝淋巴结转移瘤的MVD 为89-3 ±18-4 ,与乳腺癌组织MVD(93-8 ±21-8) 差异无显著性,且微血管分布不均,周围高于中央。结论　淋巴结微转移灶无血管生成,转移瘤有血管生成。为抑制微转移灶发展成转移瘤,以及抑制转移瘤的生长,抑制血管生成可能是控制淋巴结转移的有效措施。Objective To study angiogenesis ofaxillarylymph node metastases (including micrometastatic foci and metastatic tumors) in breast carcinoma and the relationship between microvessel density ( MVD) and metastasis. Methods Thirty seven breast cancer tissues and 121 metastatic axillary lymph nodes were collected from breast cancer patients and studied im munohistochemically. MVDwas counted by means of microvideo system under 100 ×magnification .The diameter of each micrometastasis was measured with a micrometer.Results The mean diameter of 13 micrometastatic foci was 210 ±37 μm . No blood vessel formation was found . MVDof the primarytumor and that of metastatic tumor in the axillary lymph node was 93 .8 ±21 .8 and 89 .3 ±18 .4 , respectively ( P> 0 .05) .The disribution of microvessels ofthe metastatictumorin thelymph node and thatofthe primarytumor was similar,being higher atthe periphery than atthe center.Conclusion Micrometastatic foci of breast carcinoma in the axillary lymph node do not have new blood vessel formation . Their further growth , however,depends on neo angiogenesis. Treatment based on inhibition of angiogenesis may be efficacious in the prevention of micrometastaticfocifrom developing into metastatic tumorin lymph node .

关 键 词：乳腺癌 淋巴结转移 血管生成 免疫组织化学 

分 类 号：R737.902[医药卫生—肿瘤] R733.4[医药卫生—临床医学]