TNF-a和LSA浓度联合分析对肝癌诊断和鉴别诊断的临床价值  

CLINICAL VALUES OF COMBINED DETERMINATION OF TNF-a AND LSA IN DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS OF HEPATOCELLULAR CARCINOMA

作　　者：邱历伟[1] 姚登福[1] 吴信华[1] 陆建新[1] 吴玮[1] 蒋道荣[2] 

机构地区：[1]南通医学院附属医院临床分子生物学中心,南通226001 [2]南通医学院附属医院,南通226001

出　　处：《南通医学院学报》1999年第4期380-381,共2页ACTA Academiae Medicinae Nantong

基　　金：江苏省科委项目部分资助!( BS980 2

摘　　要：目的 :观察肿瘤坏死因子 -a( TNF-a)和脂质结合唾液酸 ( LSA)在慢性肝病中的变化及其临床价值。方法 :以酶联免疫吸附法 ( EL ISA)和化学抽提法 ,分别定量检测 16例慢性肝炎、16例肝硬化和 75例肝癌患者血清 TNF -a和 L SA浓度 ,并进行了比较分析。结果 :慢性肝病患者血清 TNF和 L SA浓度均增加 ,明显高于正常对照组 ( P<0 .0 1)。在慢性肝炎、肝硬化和肝癌患者中 ,TNF-a异常率分别为 3 1.3 %、43 .8%和 93 .3 % ;L SA异常率分别为 2 5 .0 %、6 .3 %和 73 .3 % ;在肝癌患者中两者的总阳性率达 97.3 %。结论 :慢性肝病时肝细胞合成的 TNF-a和 L SA增加 。Objective :To explore the alteration and clinical values of tumor necrosis factor-a (TNF-a) and lipid associated sialic acid (LSA) in patients with chronic liver diseases. Methods: The levels of TNF-a and LSA in sera of patients with chronic hepatitis (CH), liver cirrhosis (LC) and hepatocellular carcinoma (HCC) were quantitated by enzyme-linked immunosorbent assay (ELISA) and chemical extraction assays, respectively. Results: The levels of TNF-a and LSA were significantly higher in patients with chronic liver diseases than those in normal controls (P<0.01). The positive rates of TNF-a and LSA abnormality were 31.3% and 25.0% in CH, 43.8% and 6.3% in LC, and 93.3% and 73.3% in HCC, respectively. The positive rate of combined determination of TNF-a and LSA was 97.3% in HCC. Conclusions: The present data suggest that the syntheses of TNF-a and LSA increase in hepatocytes of patients with chronic liver diseases, and the combined determination of TNF-a and LSA levels is helpful to diagnosis and differential diagnosis of HCC.

关 键 词：肿瘤坏死因子 脂质结合唾液酸 肝癌 诊断 

分 类 号：R735.704[医药卫生—肿瘤]