天然植物成份抗癌抗突变作用的分子机制研究  

Molecular mechanism of antimutagenicity of wheat sprout extract

作　　者：李佳慧[1] 高爱玲 付松滨[3] 李璞[3] 刘志诚[3] 

机构地区：[1]中国医学科学院中国协和医科大学实验动物研究所,北京100021 [2]临汾地区人民医院,山西临汾041000 [3]哈尔滨医科大学,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》1999年第6期430-432,共3页Journal of Harbin Medical University

基　　金：黑龙江省自然科学基金

摘　　要：以天然植物成份麦芽提取液为受试物，以肿瘤抑制基因Ｐ５３ 为靶基因，利用现代分子生物学技术手段，用已知序列Ｐ５３ 引物，进行ＰＣＲ扩增，获得特定的ＤＮＡ片段测序，从而对麦芽提取液抗癌抗突变作用的分子机制进行了探讨。Wheat sprout extract,a naturally occurring plant ingredient is easily prepared.There are indications that wheat sprout extract contained some antimutagenic factors.But,a molecular mechanism study of wheat sprout extract is not reported yet at home and abroad.In present experiment,the P53 gene(tumor suppressor gene) was analysed with wheat sprout extract.Total DNAs were amplified by P53 primers of known sequence.The PCR amplification products were analysed by direct sequencing.

关 键 词：麦芽提取液 植物成分 抗突变作用 分子机制 

分 类 号：R979.1[医药卫生—药品] R962[医药卫生—药学]