食管癌DNA倍体异质性肿瘤的P^(53)和ras P^(21)蛋白的表达  

Expressions of P 53 and ras P 21 protein of DNA ploidy heterogenity tumor in esophageal carcinoma

作　　者：高凤兰 原志庆[2] 千新来[2] 

机构地区：[1]漯河市卫校病理学教研室,河南漯河462000 [2]新乡医学院病理教研室

出　　处：《新乡医学院学报》1999年第4期326-326,共1页Journal of Xinxiang Medical University

摘　　要：目的　在ＤＮＡ含量研究的基础上，进一步从蛋白质表达水平上探讨ＤＮＡ 倍体异质性肿瘤的生物学特性。方法　应用流式细胞术和细胞免疫荧光技术，对１５ 例异质性肿瘤和非异质性肿瘤细胞的Ｐ５３ 和ｒａｓＰ２１ 蛋白表达量进行了定量和比较。结果　异质性肿瘤细胞的Ｐ５３ 和ｒａｓＰ２１ 蛋白的表达量显著高于非异质性肿瘤和正常组织（ Ｐ＜ ０．０５ ～０．０１）。结论　ＤＮＡ 倍体异质性肿瘤的癌基因激活和抑癌基因失活的程度高于非异质性肿瘤，异质性肿瘤的恶性程度高于非异质性肿瘤。Objective To research for biolgical charecteristics of DNA ploidy heterogenity tumor(PHT) at the level of protein,besed on the study of DNA content. Methods P 53 and ras P 21 protein expression of the PHT in 15 cases and the NPHT(non-PHT) in 15 cases were quantitatively studied and compared by flow cytometry and immunofluorecence method. Results P 53 and P 21 protein expression in PHT were higher than those in NPHT and normal tissue(P<0.05～0.01). Conclusion The extent of oncogene activation and antioncogene inactivation in PHT is much grater than that in NPHT , which shows the malignant degree of PHT is much higher than that of NPHT.

关 键 词：食管癌 DNA 倍体异质性 P^53 RASP^21 

分 类 号：R735.1[医药卫生—肿瘤]