5-氟尿嘧啶-Fe/Fe3O4磁性脂质体的制备及其表征  

Preparation and characterization of 5-fluorouracil-Fe/Fe_3O_4 magnetic liposome

作　　者：黄波涛[1] 段磊[2] 吴小玲[2] 史慧慧[2] 杨再兴[3] 钟伟[3] 戚晓红[1] 

机构地区：[1]南京医科大学病理生理系,江苏南京210029 [2]南京医科大学生物医学工程系,江苏南京210029 [3]南京大学物理系,江苏南京210008

出　　处：《南京医科大学学报（自然科学版）》2011年第7期951-955,1006,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家863子课题合作项目(2007AA021805);江苏省高校自然科学研究计划项目(08KJB310006)

摘　　要：目的:制备以Fe/Fe3O4核壳结构纳米颗粒(Fe/Fe3O4 core-shell structural magnetic nanoparticles,FCSN)为磁介质的5-氟尿嘧啶(5-FU)磁性脂质体,并检测其表征。方法:使用Fe3O4纳米颗粒通过还原氧化法制备FCSN,用透射电镜(TEM)、X射线衍射仪(XRD)及振动样品磁强计(VSM)检测其形态、结构和磁性。采用逆向蒸发法制备5-FU-FCSN磁性脂质体,通过L9(34)正交表试验得出5-FU-FCSN磁性脂质体最佳配方。用激光粒度分析仪(PCS)、TEM检测5-FU-FCSN磁性脂质体表征,并用凝胶层析法测定包封率。结果:FCSN粒径平均为70 nm,呈类圆形。XRD和TEM结果显示FCSN结构:外壳为Fe3O4,内核为Fe,磁饱和强度为107.54 emu/g。5-FU-FCSN磁性脂质体在TEM下观察为类圆形,平均粒径为202.5 nm,包封率为33.5%。放置在4℃冰箱中,1个月后包封率较为稳定,瓶底无磁性脂质体沉淀。结论:成功制备了5-FU-FCSN磁性脂质体,制备方法简单易行,制备的磁性脂质体在4℃冰箱中可以长期储存。Objective：To prepare 5-fluorouracil（5-FU） magnetic liposome with Fe/Fe3O4 core-shell structural magnetic nanoparticles（FCSN） and analysis its characteristics.Methods：Fe3O4 nanoparticles were used to prepare FCSN by reduction-oxidation method.The characteristics of FCSN were determined by X-ray diffraction（XRD）,transmission electron microscopy（TEM） and vibrating specimen magnetometer（VSM）.Using reverse-evaporation method to prepare 5-FU-FCSN magnetic targeted liposome and the best formula of magnetic liposome was obtained through the four factors of three levels orthogonal experiment.The characteristics of magnetic liposome were measured with particle size analyzer（PCS） and TEM.Entrapment efficiency was determined by gel-chromatography.Results：FCSN was similar to a globe with the average particle size of 70 nm.XRD and TEM observation of FCSN shown that its shell was Fe3O4 and kernel was Fe,magnetic saturation intensity was 107.54 emu/g.5-FU-FCSN magnetic liposome was similar to a globe with the average particle size of 202.5 nm,the entrapment efficiency of 33.5%.5-FU-FCSN magnetic liposome was relatively stable in 4℃ refrigerator after a month.Conclusion：5-FU-FCSN magnetic liposome was successfully prepared,which can be stored in 4℃ refrigerator for a long time.

关 键 词：5-氟尿嘧啶 Fe/Fe3O4纳米颗粒 磁性脂质体 

分 类 号：R730.53[医药卫生—肿瘤]