肿瘤远处转移与血浆DNA水平相关性研究  

Association of tumor metastasis with plasma DNA levels

作　　者：耿雁[1] 潘世扬[1] 王芳[1] 徐婷[1] 陆雅春[1] 彭蘡[1] 秦雪君[1] 陈丹[1] 谢而付[1] 高丽[1] 夏文颖[1] 黄珮珺[1] 黄蕾[1] 

机构地区：[1]南京医科大学第一附属医院医学检验科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2011年第8期1157-1160,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(30972821;30901344;309-01262);江苏省实验诊断学重点实验室基金(XK200731)

摘　　要：目的:探讨肿瘤转移与血浆DNA水平之间的关系,评价早期诊断肿瘤转移的方法。方法:将人肺腺癌SPC-A1细胞接种到BALB/c小鼠腹腔,每周定期检测血浆DNA水平,7周后处死小鼠,观察肿瘤形成及转移情况。结果:小鼠血浆DNA水平呈升高趋势的占75.6%(34/45只);其中接种后第3周起血浆DNA水平均比接种后第1周显著升高(P﹤0.05)。血浆DNA水平升高及持续升高组小鼠中有肺转移瘤形成的占73.5%(25/34只)。无升高组小鼠中有肺转移瘤形成的占27.3%(3/11只)。血浆DNA水平升高组及持续升高组均显著高于无升高组(P﹤0.05)。血浆DNA水平与肿瘤负荷正相关(R2=0.8703)。结论:形成肺转移瘤的BALB/c小鼠血浆DNA水平在早期即显著升高,血浆DNA水平可用于肿瘤转移的早期监测。Objective：To study the relationship between plasma DNA levels and metastasis of tumors,evaluate the method of early diagnosis of cancer metastasis.Methods：SPC-A1 were injected into BALB / c mice through the peritoneal cavity.We detected the lev-els of plasma DNA once a week.Tumors were harvested 7 weeks after tumor cells’ injection,and the metastasis conditions of tumors were also observed.Results：The percentage of mice with plasma DNA increased was 75.6%（34 / 45）,the plasma DNA levels at 3rd weeks and subsequent time points after injection were significantly higher than that at the first week（P 〈 0.05）.Among the mice in group with increased or continued increased plasma DNA,73.5%（25 / 34） were found lung cancer.However,in the plasma DNA lev-els non-increased group,27.3%（3 / 11） were found lung cancer.Statistical analysis showed that plasma DNA levels of continued in-creased group and increased group was significantly higher than those of non-elevated group（P 〈 0.05）.The levels of plasma DNA correlate with tumor burden（R 2 = 0.8703）.Conclusion：The levels of plasma DNA increased significantly at an early stage of tumors with the metastasis,the levels of plasma DNA could be used for early detection of tumor metastasis.

关 键 词：小鼠 SPC-A1 肺癌 血浆DNA 

分 类 号：R730.43[医药卫生—肿瘤]