白血病细胞体外药敏试验的临床意义及其与m dr1表达关系的探讨  

CLINICAL SIGNIFICANCE OF IN VITRO DRUG SESITIVITY ASSAY ∶RELATIONSHIP WITH MDR1 GENE EXPRESSION

作　　者：韩晓凤[1] 王燕婷[1] 韩洁英[1] 陈芳源[1] 缪金明[1] 邵念贤[1] 欧阳仁荣[1] 

机构地区：[1]上海第二医科大学附属仁济医院,200001

出　　处：《白血病》1999年第6期326-329,共4页

摘　　要：目的　探讨白血病细胞体外药敏试验的临床意义及从敏感性和耐药性两个不同侧面，研究两者内在联系。方法　采用ＭＴＴ法对２７例急性白血病患者的白血病细胞进行了Ａｒａ－Ｃ、ＤＮＲ、Ｈａｒ、ＭＩＴ、ＶＰ１６、ＶＭ２６、ＶＣＲ及ＨＣ等８ 种常用化疗药物的体外敏感试验以及用反转录－聚合酶链反应（ＲＴ－ＰＣＲ）方法检测了２２ 例患者多药耐药基因ｍ ｄｒ１ 的表达情况。结果　回顾性研究显示ＭＴＴ法体外药敏试验结果与临床疗效的总符合率为７７．３％ ，阳性符合率６１．５％ ，阴性符合率１００％ ，灵敏度１００％ ，特异度６４．３％ 。结论　ＭＴＴ法有一定的临床预测价值，是辅助临床用药选择的实验检测。ＭＤＲ相关药物ＤＮＲ、Ｈａｒ、ＭＩＴ、ＶＰ１６ 及ＶＭ２６ 体外药敏结果与ｍ ｄｒ１ 表达水平无相关性，提示可能存在其它的耐药机制。Objective To evaluate the clinical significance of in vitro drug sensitivity assay and try to find out the intrinsic relationship between sensitivity and resistance.Methods We determide in vitro drug sensitivity to Ara C、DNR、Har、MIT、VP 16 、VM 26 、VCR and HC in 27 patients with acute leukemia using the colorimetric MTT assay.The mdr1 gene expression was measured in 17 of these patients by RT PCR.Results Retrospective study showed true positive rate 61.5%,true negtive rate 100%;predictive accuracy 77.3%.Conclusion The MTT assay might serve as a reliable tool for the selection of effective anti cancer drugs in leukemia patients.There was no significant correlation between the results of the MTT assay and mdr1 gene expression,Suggesting that other drug resistance mechanisms may be involved.

关 键 词：药敏试验 MTTI地 多药耐药基因 白血病 

分 类 号：R733.7[医药卫生—肿瘤]