PTD-OD-HA融合蛋白对BALB/c裸鼠皮下K562细胞实体瘤的治疗作用  

Curative Effect of PTD-OD-HA Fusion Protein on Subcutaneous Solid Tumor Caused by K562 Cells in Nude BALB/c Mice

作　　者：季茂胜[1] 黄峥兰[1] 李亚娟[1] 黄世峰[1] 曾建明[1] 刘钉宾[1] 曹唯希[1] 冯文莉[1] 

机构地区：[1]重庆医科大学临床血液学教研室临床检验诊断学教育部重点实验室,重庆400016

出　　处：《中国生物制品学杂志》2011年第8期945-947,共3页Chinese Journal of Biologicals

基　　金：国家自然科学基金(30670901)

摘　　要：目的观察PTD-OD-HA融合蛋白对BALB/c裸鼠皮下K562细胞实体瘤的治疗作用。方法采用皮下注射K562细胞的方法建立裸鼠皮下实体瘤模型,每两天向实体瘤中间部位多点注射200μl浓度为10 mg/ml的PTD-OD-HA融合蛋白,观察裸鼠瘤体生长情况;HE染色观察肿瘤组织病理学变化;TUNEL法和透射电镜观察肿瘤组织细胞的凋亡。结果经PTD-OD-HA治疗的肿瘤体积明显缩小;肿瘤组织切片经HE染色可见细胞核浓缩和细胞染色加深;TUNEL法和透射电镜检测可见肿瘤细胞发生了凋亡的改变。结论在BALB/c裸鼠体内,PTD-OD-HA融合蛋白具有抑制K562细胞增殖和促进其凋亡的作用。Objective To observe the curative effect of PTD-OD-HA fusion protein on subcutaneous solid tumor caused by K562 cells in nude BALB / c mice.Methods Nude mouse model of subcutaneous solid tumor was established by injection s.c.with K562 cells.A portion of 200 μl of PTD-OD-HA fusion protein,at a concentration of 10 mg / ml,were injected into the middle part of tumors,at multiple sites,every 2 days.The tumors were observed for growth visually,for cell morphology by HE staining,and for cell apoptosis by TUNEL method and transmission electron microscopy.Results The sizes of tumors after treatment with PTD-OD-HA decreased significantly.HE staining showed that the nuclei were concentrated and the cells were stained darkly.TUNEL method and transmission electron microscopy showed apoptosis of the tumor cells.Conclusion PTD-OD-HA fusion protein inhibited the proliferation and promoted the apoptosis of K562 cells in nude BALB / c mice.

关 键 词：慢性粒细胞白血病 融合蛋白 BCR/ABL融合基因 小鼠 裸 

分 类 号：R730.54[医药卫生—肿瘤] R733.7[医药卫生—临床医学]