表皮生长因子受体survivn和异质性胞核核糖核蛋白A2／B1在肺癌纤维支气管镜刷片细胞学诊断中的价值  

Expression of EGFR, survivn and hnRNPA2/B1 in bronchial brushing cytology specimens and their significance in diagnosis of lung cancer

作　　者：卢珊珊[1] 潘秦镜[1] 赵琳琳[1] 郭会芹[1] 张智慧[1] 曹箭[1] 

机构地区：[1]中国医学科学院北京协和医学院肿瘤医院病理科细胞学室,100021

出　　处：《中华肿瘤杂志》2011年第8期621-625,共5页Chinese Journal of Oncology

摘　　要：目的探讨表皮生长因子受体（EGFR）、survivn和异质性胞核核糖蛋白A2和B1（hnRNPA2／B1）在肺癌纤维支气管镜刷片细胞学诊断中的价值。方法收集纤维支气管镜刷片细胞学临床诊断剩余标本197例，免疫细胞化学两步法（Envision）染色，检测EGFR、survivn和hnRNPA2／B1在非小细胞肺癌（NSCLC）、小细胞肺癌（SCLC）及非肺癌组织中的表达情况。结果EGFR、survivn和hnRNPA2／B1在NSCLC中表达率分别为81．1％、66．2％和90．9％，在非肺癌组织中的表达率分别为30．3％、3．3％和66．7％，差异均有统计学意义（均P〈0．05）；hnRNPA2／B1在SCLC表达率为92．3％，与非肺癌组织比较，差异有统计学意义（P〈0．05）。EGFR的表达与肿瘤分化程度、淋巴结转移和TNM分期有关（P〈0．05），hnRNPA2／B1表达与TNM分期有关（P〈0．05）。NSCLC和非肺癌组织中，EGFR和survivn共同表达的差异有统计学意义（98．0％和2．0％，P=0．000），EGFR和survivn均不表达的差异亦有统计学意义（38．2％和61．8％，P=0．000）。结论EGFR、survin和hnRNPA2／B1在肺癌纤维支气管镜刷片细胞学诊断中有较高的敏感性和特异性。EGFR与survivn联合应用可以提高细胞学诊断的准确性。EGFR阳性表达与肺癌患者的预后有关。Objective To study the expression of EGFR, survivn and hnRNPA2/B1 proteins in ThinPrep bronchial brushing cytology specimens and their significance in diagnosis of lung cancer. Methods The protein expression of EGFR, survivn and hnRNPA2/B1 was detected by immunocytochemistry （ICC） in the specimens from 110 cases of non-small cell lung cancer （NSCLC） , 32 cases of small cell lung cancer （SCLC） and 37 cases of non-neoplastic lung lesions. The relationship between EGFR, survivn and hnRNPA2/B1 protein expression and clinical characteristics of the patients was analyzed using SPSS 16.0 software. Results （1） Positive expression was observed in 81. 1% of NSCLC for EGFR, 66.2% for survivn, 90.9% for hnRNPA2/B1, significantly higher in NSCLC than in the control specimens （P = 0.000, P = 0. 000, P = 0. 010）. The positive expression rate of hnRNPA2/B1 in SCLC was 92.3%, significantly higher than that in the control specimens （ P = 0. 021 ）. （ 2 ） The expression of EGFR was associated with differentiation （ P = 0. 003 ）, clinical stage （ P = 0. 023 ） and lymph node metastasis （ P = 0.038）, but was not associated with gender, age and histological types. The survivn expression was not related with the above mentioned clinicopathological features （ P 〉 0.05 ）. Expression of hnRNPA2/B1 was associated with clinical stage （P = 0.017 ）, but not associated with gender, age, histological type, differentiation and lymph node metastasis. （3） There was a significant difference between the co-expression of EGFR and survivn in NSCLC （98.0%） and benign conditions （2.0%, P =0. 000）, also a significant difference between the negative expression of both EGFR and survivn in NSCLC （38. 2% ） and nonneoplastic lesions （61.8% , P = 0. 000）. Conclusions Analysis of EGFR, survivn and hnRNPA2/B1 expression may be an useful adiunct method to stratify controversial cases. The positive expression of EGFR might be associated with invasion, progression and poor prognosis of NSCLC.

关 键 词：肺肿瘤 免疫组织化学 表皮生长因子受体 Survivn HNRNPA2/B1 诊断 

分 类 号：R734.2[医药卫生—肿瘤]