美兰和超氧化物歧化酶对变形链球菌产酸作用的影响  

Effect of methylene blue and superoxide dismutase on the acids production metabolism of Streptococcus mutans

作　　者：王成龙[1] 邓斌[1] 李华[1] 苏东华[1] 

机构地区：[1]解放军总医院口腔内科,北京100853

出　　处：《临床口腔医学杂志》2011年第8期466-468,共3页Journal of Clinical Stomatology

摘　　要：目的:研究美兰(methylene blue)和超氧化物歧化酶(superoxide dismutase,SOD)对变形链球菌产酸作用的影响,探讨美兰和超氧化物歧化酶用于龋病预防的可行性。方法:采用气相色谱法研究美兰及超氧化物歧化酶对变形链球菌产酸的影响作用。用乳酸脱氢酶试剂盒检测不同培养条件下变形链球菌乳酸脱氢酶活性。结果:美兰对变形链球菌产酸具有抑制作用;SOD对变形链球菌产酸没有影响;不同培养条件下变形链球菌乳酸脱氢酶活性变化不明显。结论:由于作用机理不同,美兰和超氧化物歧化酶对变形链球菌产酸的作用也不同,美兰可能成为有价值的防龋制剂。Objective：To study the effect of methylene blue and superoxide dismutase on the acids production metabolism of Streptococcus mutans,and investigate the practicability of methylene blue and superoxide dismutase used as dental caries prevention agent.Method：Gas chromatography was used to study the effect of methylene blue and superoxide dismutase on the acids production metabolism of Streptococcus mutans in this study.The activity of lactate dehydrogenase of Streptococcus mutans incubated with different condition was determined by lactate dehydrogenase Kit.Result：Methylene blue inhibit the acids production metabolism of Streptococcus mutans,but the superoxide dismutase has no effect on the acids production metabolism of Streptococcus mutans.The action of lactate dehydrogenase of Streptococcus mutans incubated with different condition were no change obviously.Conclusion：Because of the different in role mechanism,the effect of methylene blue and superoxide dismutase on the acids production metabolism of Streptococcus mutans is different.Methylene blue may be valuable dental caries prevention agent.

关 键 词：美兰 超氧化物歧化酶 链球菌 变异 产酸 

分 类 号：R781.1[医药卫生—口腔医学] R780.2[医药卫生—临床医学]