miR-199a-3p对前列腺癌细胞迁移及侵袭能力的影响  

Effects of miR-199a-3p on Cell Migration and Invasion in Prostate Cancer Cells

作　　者：孔繁飞[1] 王中显[1] 孙朝阳[1] 吕煊[1] 翁丹卉[1] 卢运萍[1] 陈刚[1] 吴明富[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科肿瘤生物医学中心,武汉430030

出　　处：《肿瘤防治研究》2011年第8期875-877,共3页Cancer Research on Prevention and Treatment

基　　金：国家科技部973计划基金资助项目(2009CB521808);教育部新教师基金资助项目(200804871056);湖北省卫生厅青年科技人才基金资助项目(QJX2010-3)

摘　　要：目的从miR-199a-3p在前列腺癌高、低转移潜能细胞中的表达差异出发,研究其在高转移前列腺癌细胞株1E8细胞运动转移中的作用及可能的分子机制。方法运用Realtime PCR法检测miR-199a-3p在前列腺癌高、低转移潜能配对细胞系中的表达差异。通过划痕实验及transwell实验观察1E8细胞及转染miR-199a-3p mimics及mimics NC后该细胞运动迁移能力的变化。结果 (1)RealtimePCR结果显示高转移潜能1E8细胞中miR-199a-3p表达水平明显低于低转移潜能2B4细胞。(2)上调miR-199a-3p会减弱1E8细胞的运动转移能力。结论 miR-199a-3p的上调可以抑制前列腺癌细胞的运动迁移能力。Objective To investigate the different expression of miR-199a-3p in different prostate cancer cell lines and the effects of miR-199a-3p on cell migration and invasion in prostate cancer.Methods The expression of miR-199a-3p in 1E8（high metastasis prostate cancer） and 2B4（low metastasis prostate cancer） cell lines were identified by realtime PCR.The migration and invasion abilities were detected by Transwell experiment.Results（1）By comparing with 2B4 cells,of miR-199a-3p was decreased in 1E8 cells.（2）The invasiveness of 1E8 cells were decreased through miR-199a-3p upregulation.Conclusion miR-199a-3p invasiveness of prostate cancer cells.

关 键 词：前列腺癌 miR-199a-3p MICRORNA 

分 类 号：R737.25[医药卫生—肿瘤]