YC-1对人肝细胞癌裸鼠移植瘤的影响及其机制  

Mechanism of Effects of YC-1 on Human Primary Hepatic Carcinoma Loaded in Nude Mice

作　　者：吴晓慧[1] 王顺祥[1] 杨永江[1] 李建坤[1] 

机构地区：[1]河北医科大学第四医院肝胆外科,石家庄050011

出　　处：《肿瘤防治研究》2011年第8期895-898,共4页Cancer Research on Prevention and Treatment

基　　金：河北省卫生厅基金资助课题(05012)

摘　　要：目的探讨缺氧诱导因子抑制剂(YC-1)对人肝癌细胞裸鼠皮下移植瘤的影响及其相关机制。方法用人肝癌细胞株SMMC-7721建立人肝癌裸鼠皮下移植瘤模型,待肿瘤生长至约(100～150)mm3时,将荷瘤裸鼠随机分为两组:实验组和对照组,分别给予YC-1和二甲基亚砜,观察两组裸鼠肿瘤生长情况;应用RT-PCR、Western blot及免疫组织化学方法检测移植瘤组织HIF-1α和VEGF表达。结果 YC-1治疗组各时点肿瘤体积显著低于对照组(P<0.05);与对照组比较,实验组移植瘤组织HIF-1αmRNA表达差异无统计学意义(P>0.05),而HIF-1α蛋白的表达显著降低(P<0.05);移植瘤组织VEGFmRNA及蛋白表达均显著低于对照组(P<0.05)。结论 YC-1可能通过下调HIF-1α和VEGF的表达来抑制肝癌的生长。Objective To research the mechanism of effects of HIF-1α（YC-1） on implanted human primary hepatic carcinoma in nude mice.Methods A total of 16 male nude mice were inoculated subcutaneously with 1 million SMMC-7721 cells.Once the tumor grew to（100~150）mm3,the mice were divided randomly into two groups and injected with YC-1 or DMSO respectively.Tumor size and weight were measured.Levels of HIF-1α and VEGF expressions in tumor tissue were detected by RT-PCR,Westetn blot or immunohistochemical respectively.Results Tumors grew rapidly in the control group than those in the YC-1 treatment group.In YC-1 group therapy resulted in low expression of HIF-1α and VEGF than those in control groups（P0.05）.Conclusion YC-1 therapy was a promising approach to treat human liver cancer by suppression of HIF-1α and VEGF expression,which might contribute to inhibit tumor growth and angiogenesis.

关 键 词：YC-1 缺氧诱导因子-1Α 血管内皮生长因子 肝细胞癌 裸鼠 

分 类 号：R735.7[医药卫生—肿瘤]