PKC-ζ在大肠良恶性组织中的表达及其与Cortactin蛋白的关系  

Prognostic Significance of PKC-ζ and Cortactin Expression in Colorectal Carcinoma

作　　者：陈曦[1] 毛勤生[1] 黄华[2] 朱建伟[1] 

机构地区：[1]南通大学附属医院普通外科,江苏南通226001 [2]南通大学附属医院病理科,江苏南通226001

出　　处：《肿瘤防治研究》2011年第8期903-908,共6页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30771126;30772106);教育部回国留学人员基金资助项目(2008-890);江苏省自然科学基金资助项目(BK2006058)

摘　　要：目的探讨蛋白激酶C-ζ(PKC-ζ)、皮质肌动蛋白(Cortactin)在大肠癌组织、癌前病变组织(腺瘤性息肉)、正常组织中的综合表达及其意义。方法采用免疫组织化学SP法,检测三种不同组织中PKC-ζ和Cortactin的表达,分析PKC-ζ、Cortactin在大肠癌中的表达意义、与临床病理因素的关系及其两者表达的相互关系。结果 (1)PKC-ζ和Cortactin表达均数大肠癌组织比大肠腺瘤组织及正常组织高,差异有统计学意义(P<0.05);(2)PKC-ζ与Cortactin的表达均与肿瘤分化程度、浸润深度、淋巴结转移和TNM分期有关(P<0.05),而与性别、年龄、肿瘤部位、大小和组织类型无关(P>0.05);(3)PKC-ζ的阳性表达与Cortactin的阳性表达正相关(r=0.574,P<0.05)。结论 PKC-ζ和Cortactin可作为大肠癌预后判断及肿瘤分期监测的有价值的指标。联合检测PKC-ζ和Cortactin对预测大肠癌的侵袭转移潜能及评估大肠癌病人的预后有重要意义。PKC-ζ与Cortactin可能作为大肠癌检测的肿瘤标记物和治疗的分子靶点。Objective To investigate the relationship between in colorectal cancer,precancerous lesions（adenomatous polyps）,and normal tissues and analyze the prognostic impact of PKC-ζ and Cortactin in colorectal cancer. Methods The expressions of PKC-ζ and Cortactin were examined by SP immunohistochemistry in colorectal cancer,colorectal adenomas and colorectal tissues adjacent to adenocarcinomas（normal tissues）.Results（1）The positive rates of PKC-ζ and Cortactin in colorectal cancer tissues were significantly higher than those of colorectal tissue adjacent to adenocarcinomas（normal tissues） and colorectal adenomas（P0.05）.（2）There was a significant correlation between PKC-ζ and Cortactin expression and tumor cell differentiation,invasive depth,lymph node metastasis and TNM stage（P0.05）,but no statistically correlation between PKC-ζ and Cortactin expression and the age,gender,localization of tumor,tumor size and histological types（P0.05）.（3）There was a positive correlation between PKC-ζ and Cortactin（r=0.574,P0.05）.Conclusion PKC-ζ and Cortactin might be valuable markers in predicting the prognosis of the colorectal cancer and molecule targets for therapy.The combined detection of the PKC-ζ and Cortactin might be a more appropriate method to identify invasion and metastasis and predict the prognosis of colorectal cancer.

关 键 词：大肠癌 蛋白激酶C-ζ 皮质肌动蛋白 免疫组织化学 

分 类 号：R730.2[医药卫生—肿瘤] R735.34[医药卫生—临床医学]