SIAH2与Bcl-2在乳腺癌中的表达和相关性研究  

Expression and Correlation of SIAH2 and Bcl-2 in Breast Cancer

作　　者：甄娟[1] 李照青[2] 刘玉东[3] 李栢林[4] 宋敏[1] 

机构地区：[1]中国医科大学附属第一医院病理科,中国医科大学基础医学院病理教研室,沈阳市110001 [2]北京大学人民医院内分泌科 [3]中国医科大学附属第一医院耳鼻喉科 [4]中国医科大学基础医学院化学教研室

出　　处：《中国肿瘤临床》2011年第15期898-901,共4页Chinese Journal of Clinical Oncology

摘　　要：目的:分析正常乳腺组织和浸润性导管癌中SIAH2和Bcl-2的表达及临床意义,探讨其在乳腺癌发生发展中的作用及相关性。方法:采用免疫组织化学S-P法检测SIAH2和Bcl-2蛋白的表达,并分析二者的相关性及与临床病理因素的关系;采用Western Blot法检测SIAH2和Bcl-2蛋白的表达。结果:SIAH2与Bcl-2蛋白在乳腺癌癌变过程中的阳性表达率逐渐升高,并且二者表达与乳腺癌组织学分级、ER、PR相关。免疫组化结果表明SIAH2与Bcl-2蛋白在乳腺正常组织和浸润性导管癌中的阳性表达率分别为8.7%和17.4%,75%和72.5%。统计学结果显示在80例乳腺癌组织中,SIAH2与Bcl-2蛋白表达呈正相关(P<0.001,r_s=0.485)。Western blot结果表明SIAH2与Bcl-2蛋白在乳腺癌组织中表达高于癌旁乳腺组织,在乳腺癌细胞系MDA-MB-435S和MCF-7中表达高于乳腺正常上皮细胞系MCF-10A。结论:SIAH2与Bcl-2在乳腺癌发生发展中均存在异常,并且两者可能存在某种协调关系。Objective： To investigate the expression and significance of both seven in absentia homology 2 （ SIAH2 ） and B-cell lymphoma 2 （ Bcl-2 ） in normal mammary glands and invasive ductal carcinoma （ IDC ） and to discuss the role and correlation between them during breast carcinogenesis. Methods： SIAH2 and Bcl-2 expression were determined using immunohistochemical SP method, and the relationship between the SIAH2 and Bcl-2 protein expression and the clinical pathologic features were analyzed. Western blot analysis was conducted to determine the SIAH2 and Bcl-2 expression. Results： SIAH2 and Bcl-2 expression significantly increased during breast carcinogenesis and were correlated with histologic grade, as well as estrogen and progesterone receptor expression. The immunohistochemistry results showed that there was an increasing tendency in positive expression rate of SIAH2 and Bcl-2 in normal breast tissues （ 8.7% and 17.4%, respectively ） to IDC （7 5% and 72.5%, respectively ）. Spearman＇s correlation revealed that SIAH2 expression was significantly correlated with Bcl-2 expression in the 80 breast cancer specimens （ P 〈 0.001, rs = 0.485 ）. Western blot analysis showed that SIAH2 and Bcl-2 protein expression were significantly higher in breast tumor tissues compared with those in paraneoplastic tissues, which was the same compared with the protein expression among the human breast cancer MDA-MB-435S and MCF-7 cell lines, and the normal breast epithelial MCF-10A cell line. Conclusion： Our results indicated that both SIAH2 and Bcl-2 are involved in breast cancer and may imply coordination in breast tumorigenesis.

关 键 词：SIAH2 BCL-2 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]