siRNA下调TROP-2基因表达对人胰腺癌细胞迁移和侵袭的影响  

Effects of TROP-2 down-regulation by small interfering RNA on migration and invasion of human pancreatic cancer cell

作　　者：瞿彩兴[1] 马圭[1] 范钰[2] 

机构地区：[1]江苏大学附属人民医院普外科,江苏镇江212002 [2]江苏大学附属人民医院,肿瘤研究所,江苏镇江212002

出　　处：《中国肿瘤外科杂志》2011年第4期201-203,223,共4页Chinese Journal of Surgical Oncology

基　　金：镇江市社会发展基金(SH2009014)

摘　　要：目的探讨TROP-2基因小干扰RNA对胰腺癌细胞迁移、侵袭的影响。方法培养人胰腺癌CFPAC-1细胞株,采用TROP-2基因小干扰RNA转染胰腺癌细胞株,分别以荧光实时定量PCR和免疫荧光方法观察TROP-2基因的mRNA和蛋白水平,随后以划痕法观察癌细胞迁移、以Boyden法检测癌细胞侵袭力。结果以TROP-2 siRNA转染胰腺癌CFPAC-1细胞后,癌细胞TROP-2基因mRNA和蛋白含量明显下降,且呈时间和浓度依赖性(P<0.001;P<0.001);划痕试验和Boyden试验结果显示,细胞迁移和侵袭力均明显下降,且呈浓度依赖性(P<0.001;P<0.001)。结论 TROP-2基因可能在胰腺癌细胞迁移和侵袭中发挥着重要作用;以siRNA转染胰腺癌细胞,可抑制胰腺癌细胞的迁移和侵袭能力。Objective To study the effects of TROP-2 gene small interfering RNA （siRNA） on migration and invasion of human pancreatic cancer cell. Methods After human pancreatic cancer cell line CFPAC-1 was tasfected with different dose of TROP-2 siRNA, and the expression of TROP-2 mRNA and protein were determined by real-time quantitative PCR and immunoflurescence method. Cell migration, and invasion was exmined by wound healing, and boyden chamber, respectively. Results The results from real-time quantitative PCR and immunoflurescence method showed that TROP-2 mRNA and protein reduced in time and dose-dependent manners （P 〈 0. 001 ; P 〈 0. 001 ）. The migrative and invasive ability of CFPAC-1 cell treated with TROP-2 siRNA decreased compared with control groups （P 〈 0. 001 ; P 〈 0.001 ）. Conclusions TROP-2 gene might play an important role in migration, and invasion of human pancreatic cancer cell. siRNA targeted TROP-2 could ef- fectively inhibit migration and invasion of human pancreatic cancer cell.

关 键 词：胰腺癌 癌细胞迁移 TROP-2 小干扰RNA 癌细胞侵袭 基因表达 

分 类 号：R735.9[医药卫生—肿瘤]