制作特殊皮肤标本石蜡组织芯片的方法  

Manufacture technique in specific skin tissue microarray

作　　者：陈先才[1] 林常敏[1] 陈海滨[1] 汪洪志[1] 蔡博治[1] 黄铿[1] 

机构地区：[1]汕头大学医学院组织胚胎学教研室,广东汕头515041

出　　处：《中国美容整形外科杂志》2011年第8期499-502,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：基金项目：国家自然科学青年基金资助项目（30801196）;广东省自然科学基金资助项目（9151008901000141）;广东省科技计划资助项目（20088030301368）

摘　　要：目的寻找一种简易、芯点损失率低的方法制作包含特殊组织（如软骨、厚角质层）皮肤标本的组织芯片。方法采用自制打孔器取34个直径为3mm的大鼠足垫组织块，制成6x6组织芯片；采用手工切割法取27个长条形大鼠耳、背皮肤组织块，制成一块9×3组织芯片；分别进行切片、常规HE染色、拍照，对比其芯点损失率。结果传统打孔器法所得组织芯片损失率为11．8％；手工切割法背部皮肤无芯点损失，鼠耳芯点损失率18．5％。结论手工切割法操作简单，得到的芯片质量完整性好，对于软骨等特殊组织及阳性面积较大的组织而言，比传统打孔器法更显优势。Objective To investigate a method of making specific skin tissue microarray including cartilage and thick corneum with easy performation and low loss rate of tissue array. Methods Totally 34 tissue pieces with 3 mm diameter were taken from rat footpad by a self-made puncher to make 6 x 6 tissue microarray; 27 strip-shaped tissue pieces from rat ear and back skin were harvested by manual cutting to make 9 ×3 tissue microarray. All the tissue microarrays were cut into slices, stained by HE, taken pictures and the tissue array loss rates between the two groups were compared. Results The loss rate of the traditional puncher method was 11.8% ; there was no loss of tissue microarray in the back skin group by manual cutting method, but 18.5% in the ear skin group. Conclusion The manual cutting is an optimal technology for specific skin tissue, especial- ly for the treatment of special tissue as cartilage or some tissue with large positive area.

关 键 词：皮肤 组织芯片 制作方法 软骨 

分 类 号：R622[医药卫生—整形外科]