ENT1在人肝癌细胞中的表达特征及临床意义  

Expression characteristics of ENT1 in human hepatocellular carcinoma cells

作　　者：蒋星星[1] 傅华群[1] 巢映辉[1] 刘征宇[2] 

机构地区：[1]南昌大学第二附属医院肝胆外科,江西省南昌市330006 [2]南昌大学医学院实验教学部,江西省南昌市330031

出　　处：《世界华人消化杂志》2011年第20期2104-2108,共5页World Chinese Journal of Digestology

基　　金：江西省卫生厅中医药科研基金资助项目;No.2009021~~

摘　　要：目的:探讨ENT1在人肝癌细胞株中的表达特征及其临床意义.方法:人肝癌细胞HuH-7、HepG2、Hep3B并选择人乳腺癌细胞MCF-7为对照,采用免疫荧光法检测细胞中ENT1的定位表达;RT-PCR法检测细胞中ENT1mRNA的表达;CDKs特异性抑制剂干预人肝癌细胞HuH-7的细胞周期,RT-PCR法检测细胞不同周期ENT1mRNA的表达特征.结果:3株人肝癌细胞膜上均可见ENT1的表达;肝癌细胞中ENT1mRNA的表达水平均高于乳腺癌细胞;不同肝癌细胞株中ENT1mRNA的表达水平存在差异(HuH-7:0.756±0.019,HepG2:0.469±0.041,Hep3B:0.580±0.030,MCF-7:0.356±0.029);肝癌细胞被阻滞在G0/G1期后,ENT1mRNA的表达水平明显上调(0.737±0.017vs0.345±0.027,P<0.05).结论:人肝癌细胞膜上普遍存在较高的ENT1表达,不同的肝癌细胞及不同的细胞周期其表达均存在差异.ENT1在人肝癌细胞中的表达特征对临床化疗策略具有潜在的临床价值,并为认识肝癌的耐药机制提供新的思路.AIM： To investigate the expression characteristics of equilibrative nucleoside transporter 1 （ENT1） in human hepatocellular carcinoma （HCC） cell lines. METHODS： Human HCC cell lines HUH-7, HepG2 and Hep3B, and the breast cancer cell line MCF7 were used in this study. ENT1 was localized by immunofluorescence staining. Before and after treatment with roscovitine, a cyclin-dependent kinase inhibitor, the expression of ENT1 mRNA in the above cell lines was detected by reverse transcription-polymerase chain reaction （RT-PCR）.RESULTS： ENT1 could be detected on the membrane of the three human HCC cell lines. The expression levels of ENT1 mRNA in HCC cell lines were higher than that in the breast cancer cell line （HUH-7：0.756 ± 0.019; HepG2：0.469 ± 0.041; Hep3B： 0.580 ± 0.030; MCF-7：0.356 ± 0.029）. The expression level of ENT1 mRNA differed significantly among the three human HCC cell lines. Treatment with roscovitine increased the expression level of ENT1 mRNA （0.737 ± 0.017 vs 0.345 ± 0.027, P 〈 0.05）. CONCLUSION： ENT1 was localized to the membrane of HCC cells. The expression levels of ENT1 mRNA vary among different HCC cell lines. Treatment with roscovitine increased the expression level of ENT1 mRNA in HCC cell lines.

关 键 词：核苷载体 肝癌细胞 平衡型核苷载体1 

分 类 号：R735.7[医药卫生—肿瘤]