COX-2与GCS在胃癌组织中的表达及意义  

Significance of expression of COX-2 and GCS in gastric cancer

作　　者：王俊先[1] 张开光[2] 李诚[1] 李祥春[2] 王晓秋[3] 丁敏[3] 

机构地区：[1]安徽省立友谊医院消化内科,安徽省合肥市230041 [2]安徽医科大学附属省立医院消化内科,安徽省合肥市230001 [3]安徽医科大学附属省立医院病理科,安徽省合肥市230001

出　　处：《世界华人消化杂志》2011年第20期2171-2175,共5页World Chinese Journal of Digestology

摘　　要：目的:研究环氧合酶2(COX-2)与多药耐药基因葡萄糖神经酰胺合成酶(GCS)在胃癌组织中的表达及其相关性,初步探讨COX-2参与胃癌多药耐药的可能机制.方法:采用免疫组织化学法对经手术切除的63例胃癌组织标本及30例非癌性胃组织标本中COX-2及GCS的表达情况进行研究,并且对COX-2与GCS在胃癌组织中的表达进行相关性分析.结果:COX-2、GCS在胃癌组织中的阳性表达均高于非癌性胃组织(87.3%vs36.7%;49.2%vs23.3%,均P<0.05).在有淋巴结转移的胃癌组织中COX-2阳性表达率高于无淋巴结转移的胃癌组织(100%vs69.2%,P<0.05),在不同年龄、性别、不同分化程度、不同的癌肿浸润深度中COX-2的阳性表达率无明显差异;GCS在胃癌组织中的阳性表达率与有无淋巴结转移、年龄、性别、分化程度、不同的侵犯深度等均无明显关系.在胃癌组织中,COX-2与GCS的表达正相关(r=0.724,P=0.004).结论:GCS在胃癌组织中存在高表达,可能是引起胃癌原发性多药耐药的耐药基因之一;在胃癌组织中,COX-2与GCS的表达存在正相关,COX-2介导胃癌耐药可能与影响GCS在胃癌组织中的表达有关.AIM： To investigate the expression of cyclooxygenase-2 （COX-2） and glucosylceramide synthase （GCS） in gastric cancer and to analyze their clinical significance. METHODS： Immunohistochemistry was used to investigate the expression of COX-2 and GCS in 63 cases of gastric cancer tissues and 30 cases of non-gastric cancer tissues. The correlation of COX-2 and GCS expression with patient＇s age, sex, tumor histological type, differentiation, and lymph node metastasis was analyzed. RESULTS： The positive rate of COX-2 expression in gastric cancer was higher than that in non-cancerous tissue （87.3% vs 36.7%, P 〈 0.05）. The positive rate of GCS expression in gastric cancer was also higher than that in non-cancerous tissue （49.2% vs 23.3%, P 〈 0.05）. Neither COX-2 nor GCS expression was related with age, sex, tumor differentiation, depth of invasion or histological type （all P 〉 0.05）. The positive rate of COX-2 in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis （100% vs 69.2%, P 〈 0.05）, whereas the positive rate of GST-П was not related with lymph node metastasis （P 〉 0.05）. There is a significant correlation between the expression of COX-2 and that of GST-П in gastric cancer （r = 0.724, P= 0.004）. CONCLUSION： GCS is highly expressed in gastric cancer and may be involved in tumor drug resistance. COX-2 may mediate drug resistance by influencing the expression of GCS in gastric cancer.

关 键 词：环氧合酶-2 葡萄糖神经酰胺合成酶胃癌 胃癌 多药耐药 相关性 

分 类 号：R735.2[医药卫生—肿瘤]