一种有效区分移植细胞和宿主细胞脑损伤模型的建立  

A traumatic brain injury model for distinguishing between transplanted neural cells and host cells in vivo

作　　者：杨上川[1,2] 董锦润[1,2] 瞿家桂[1,2,3] 胡新天[1,2] 王正波[1,2] 

机构地区：[1]中国科学院生物物理研究所脑与认知国家重点实验室,北京100101 [2]中国科学院昆明动物研究所,云南昆明650223 [3]中国科学技术大学生命科学学院,安徽合肥230026

出　　处：《Zoological Research》2011年第4期421-427,共7页动物学研究（英文）

基　　金："973"项目(2007CB947703)

摘　　要：为了区分移植神经细胞和宿主细胞,便于将来在宿主体内对移植细胞进行在体的电生理记录以及其它方面的研究,通过机械损毁的方法,建立了一种特殊的脑损伤模型。结果发现,通过机械损毁的方法,在大鼠大脑皮层形成形态规则的损伤空洞,其模型稳定,重复性好;在空洞内进行干细胞移植,能够长时间存活,移植神经干细胞绝大部分细胞分化为神经元,只有少量细胞分化为胶质细胞,而且移植细胞与宿主细胞分界明显;对移植细胞进行单细胞电生理记录,记录到神经元放电信号。这些结果表明,通过机械损毁的方法,在大鼠大脑皮层成功建立了一个稳定、精确定位移植细胞与宿主细胞界限的脑损伤模型。To perform electrophysiological recording and other investigations on transplanted neural cells in vivo,we used mechanical damage to establish a special traumatic brain injury model that could distinguish transplanted cells from host cells.The morphology of the trauma-induced holes in the cortex of the rat brain was regular.The model was stable and repeatable.Neural stem cells were transplanted into the trauma-induced hole,and were able to survive for a long time.Most of the transplanted cells differentiated into neurons,and only a small amount turned into glia cells.There was a clear boundary between the host cells and the transplanted cells.Single cell electrophysiological recording on transplanted neural cells were detected in vivo.This study established a stable and repeatable traumatic brain injury model,which could be used to conduct in vivo electrophysiological recording research on transplanted neural cells.

关 键 词：机械损毁法 移植细胞 宿主细胞 神经干细胞 脑损伤 

分 类 号：R-332[医药卫生]