淋巴瘤Raji细胞Id4基因甲基化的检测及意义  

Study on the methylation of Id4 gene in Raji lymphoma cells

作　　者：曲凡[1] 赵春华[1] 杨明娟[1] 孟建辉[1] 朱秀丽[1] 陈健[1] 李梅[1] 

机构地区：[1]河北医科大学第四医院儿科,石家庄050011

出　　处：《中国小儿血液与肿瘤杂志》2011年第4期161-163,共3页Journal of China Pediatric Blood and Cancer

基　　金：河北省卫生厅医学科学研究重点课题计划(项目编号:08347)

摘　　要：目的检测恶性淋巴瘤细胞Id4基因是否发生了甲基化。方法体外培养人Burkitt’s淋巴瘤细胞株Raji细胞,用甲基化特异性聚合酶链反应(MS-PCR)法检测Raji细胞的Id4基因甲基化状态,逆转录聚合酶链反应(RT-PCR)法检测Id4 mRNA的表达情况。结果 MS-PCR法检测显示Raji细胞的Id4基因存在高甲基化状态,RT-PCR法未检测出Id4 mRNA表达。结论人Burkitt’s淋巴瘤细胞株Raji细胞Id4基因呈现高甲基化状态,Id4基因表达沉默。Objective To detect the methylation of Id4 gene in malignant lymphoma. Methods Human Burkitt＇ s Raji lymphoma cells were cultivated in vitro. The methylation of Id4 gene was detected by Methylation Specificity Polymerase Chain Reaction （MS-PCR） in Raji cells. The mRNA expression of Id4 was detected by Reverse Transcription Polymerase Chain Reaction （RT-PCR）. Results Id4 gene presented an exhaustive methylation. No mRNA expression of Id4 was detected in Raji cells. Conclusions Id4 gene presented an exhaustive methylation and gene silencing in Raji cells. Hyperm- ethylation of Id4 gene might be one of the reasons of malignant behavior in Raji cells.

关 键 词：淋巴瘤 甲基化 ID4 特异性聚合酶链反应 

分 类 号：R733.1[医药卫生—肿瘤]