体外诱导的骨髓源肝细胞样细胞移植治疗肝功能衰竭的实验研究  

Study on the therapy of hepatic failure using hepatocyte-like cells derived from bone marrow transplantation

作　　者：刘芳[1] 陈少红[2,3] 刘青波[2,3] 范新娟[2,3] 唐芳[2,3] 刘大伟[2,3] 赵国强[2,3] 

机构地区：[1]南华大学医学院病理学教研室肿瘤细胞与分子病理学湖南省高校重点实验室,湖南衡阳421001 [2]中山大学中山医学院病理学教研室 [3]中山大学附属第一医院病理科,广州510080

出　　处：《重庆医学》2011年第24期2398-2400,F0002,共4页Chongqing medicine

基　　金：国家自然科学基金(NO:30170473);广东省自然科学基金(NO:0400932);湖南省教育厅课题(NO:08C744);湖南省重点学科建设资助项目(200-180);湖南省高校重点实验室资助项目

摘　　要：目的探讨骨髓源肝细胞样细胞移植对急性肝功能衰竭的治疗作用。方法体外诱导大鼠骨髓间质干细胞(BMSCs)分化,以BST-1、清蛋白(ALB)为分子标志,采用逆转录聚合酶链反应(RT-PCR)和流式细胞技术(FCM)检测其分化状态。构建大鼠急性肝功能衰竭模型,随机分为两组:实验组(骨髓源肝细胞样细胞移植)和对照组,结合多项血生化指标、病理形态学和生存状况综合评估骨髓源肝细胞对肝功能重建的影响。结果诱导20 d的BMSCs髓系来源标志BST-1表达消失,成熟肝细胞标志ALB的表达出现,流式细胞术检测结果亦表明其高表达ALB;骨髓源肝细胞移植可降低血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)水平,提高清蛋白合成能力,改善大鼠肝功能和生存状况。结论骨髓源肝细胞样细胞移植对肝功能恢复可以起到有效作用,可作为肝功能衰竭进行细胞移植的一个理想细胞源。Objective To explore the therapeutic effect of acute hepatic failure by transplanting hepatocyte-like cells derived from bone marrow. Methods BMSCs were differentiated in vitro. RT-PCR and flow cytometry （FCM） were performed to examine the cellular differentiated states by exploring the expression of BST-1 and albumin. Rats with acute hepatic failure model were randomly divided into 2 groups., experimental group（hepatocyte-like ceils transplantation） and control group. The levels of biochemistry,pathological morphology and survival state analysis were used to evaluate the ability of hepatocyte-like cells derived from bone marrow to improve the liver functions. Results Hepatocyte-like cells expressed the gene of albumin,but the gene of BST-1 disappeared after the induction for 20 days. The result of FCM showed that hepatocyte-like cells expressed albumin too. Hepatocyte-like cells transplantation could lower the level of AST,ALT and TBIL,enhanced the ability to synthesizing ablumin,and improved the liver functions and survival rate of acute hepatic failure rats remarkably. Conclusion Hepatocyte-like cells derived from bone marrow can improve hepatic function,which can be used for a perfect cell source of HCT.

关 键 词：间质干细胞 肝功能衰竭 急性 细胞移植 

分 类 号：R575.3[医药卫生—消化系统]