固体脂质纳米姜黄素联合顺铂对卵巢癌SKOV3的增殖及Bax/Bcl-2蛋白表达的实验研究  

Effect of curcumin-loaded solid lipid nanoparticles and DDP on proliferation and expression of Bax/Bcl-2 protein in human ovarian cell line SKOV3

作　　者：邓舒婷[1] 甘霖[2] 周琦[1] 徐建业[2] 李少林[1] 

机构地区：[1]重庆医科大学基础医学院放射医学教研室,重庆400016 [2]重庆市肿瘤研究所妇瘤科,重庆400030

出　　处：《重庆医科大学学报》2011年第6期650-653,共4页Journal of Chongqing Medical University

基　　金：重庆市卫生局科研资助项目(编号:2007-2-68)

摘　　要：目的:研究固体脂质纳米姜黄素(Curcumin-loaded solid lipid nanoparticles,SLN-Cur)与顺铂(Cisplatin,DDP)联用对人卵巢癌SKOV3细胞株生长的影响及促凋亡作用。方法:姜黄素(Curcumin,Cur)制备成SLN-Cur新型给药系统,分别检测DDP、Cur、SLN-Cur、DDP+Cur、DDP+SLN-Cur对人卵巢癌SKOV3细胞株凋亡形态学变化及超微结构变化,细胞凋亡率及凋亡相关基因蛋白的表达。结果:SLN-Cur可抑制SKOV3细胞生长,抑制率与药物浓度及作用时间成依赖关系,24 h IC50=40.14μmol/L,DDP与SLN-Cur联合诱导细胞凋亡作用增强,具有显著协同作用;Bcl-2表达减弱,Bax表达增强。结论:SLN-Cur合用DDP对SKOV3细胞株具有较好的抑制增殖及促凋亡作用,通过下调Bcl-2表达及上调Bax表达而诱导细胞凋亡。Objective：To investigate the effect of curcumin-loaded solid lipid nanoparticles（SLN-Cur）in combination with cisplatin（DDP）on growth of human ovarian cancer cell line SKOV3 and its apoptosis induction.Methods：SLN-Cur was prepared in an aqueous system using curcumin as a model drug.SKOV3 cells were treated with curcumin,DDP,SLN-Cur,curcumin combined with DDP and SLN-Cur combined with DDP respectively.Cell morphology variation and cell ultrastructure were observed;the apoptosis rate of SKOV3 cell and the expression of apoptosis-associated gene proteins were detected.Results：Curcumin-loaded solid lipid nanoparticles（SLN-Cur）could effectively inhibit SKOV3 in a dose-and-time-dependent manner.The IC50 of curcumin-loaded solid lipid nanoparticles（SLN-Cur）to SKOV3 was 40.14 μmol/L in 24 hours.Simultaneous administration of SLN-Cur and DDP resulted in synergistic cytotoxicity and apoptosis for SKOV3 cells;the expression of Bcl-2 was down-regulated while the expression of Bax was up-regulated.Conclusion：The rate of cell proliferation inhibition and the rate of the SKOV3 cell line apoptosis by SLN-Cur combined with DDP are significantly higher.The apoptosis is related to the down-regulation of Bcl-2 and up-regulation of Bax.

关 键 词：固体脂质纳米粒 姜黄素 顺铂 人卵巢癌SKOV3细胞株 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]