VEGF-α诱导对卵巢癌血管生成拟态形成及侵袭、迁移能力的影响  

Effect of VEGF-α induction on vasculogenic mimicry formation,invasion and migration ability of human ovarian carcinoma cell lines

作　　者：王俊艳[1] 王立[1] 孙涛[2] 赵秀兰[2] 赵楠[2] 孙保存[2] 

机构地区：[1]天津医科大学组织学与胚胎学教研室 [2]天津医科大学病理学教研室,天津300070

出　　处：《中国组织化学与细胞化学杂志》2011年第1期48-52,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：天津市应用基础及前沿技术研究项目(10JCYBJC12900)

摘　　要：目的探讨细胞因子VEGF-α诱导对卵巢癌SKOV-3和OVCAR-3细胞系血管生成拟态形成及侵袭、迁移能力的影响。方法采用不同细胞因子诱导卵巢癌SKOV-3和OVCAR-3细胞系,通过三维培养来观察卵巢癌细胞形成血管生成拟态的能力;以划痕实验和侵袭实验来判断细胞因子诱导对卵巢癌细胞侵袭、迁移能力的影响。结果三维培养结果表明在VEGF-α组SKOV-3和OVCAR-3细胞可观察到大量、明显的血管样网状结构;细胞划痕试验结果表明卵巢癌细胞系经细胞因子诱导后,其迁移能力明显增强;体外侵袭实验表明VEGF-α能够明显增强卵巢癌SKOV-3和OVCAR-3细胞的体外侵袭能力。结论 VEGF-α能促进卵巢癌细胞系血管生成拟态的形成,并明显增强卵巢癌细胞侵袭和迁移的能力。Objective To investigate the effect of VEGF-α on ovarian carcinoma vasculogenic mimicry formation,invasion and migration.Methods The ovarian carcinoma cell lines SKOV-3 and OVCAR-3 were induced by different cytokines.After that the three dimensional culture was used to observe VM formation.Invasion and wound healing assays were performed to judge the invasion and migration ability.Results Three-dimensional culture results demonstrated that both SKOV-3 and OVCAR-3 cells,when stimulated by VEGF-α,formed typical pipe-like structures after culture within the three-dimensional Matrigel medium.Scratch test showed that the treatment of cells with cytokines markedly enhanced the cell migration into the denuded area.Conclusion VEGF-α is regarded as the key regulation molecule in VM formation,invasion and migration,of ovarian carcinoma cell lines.

关 键 词：VEGF-α 卵巢癌 血管生成拟态 侵袭 迁移 

分 类 号：R730[医药卫生—肿瘤]