ERCC-1和RRM-1在NSCLC中的表达及其病理临床意义  

Expression of ERCC-1 and RRM-1 and its relationship with clinical pathological characteristics in non-small cell lung cancer

作　　者：李明显[1] 夏传生[2] 李娜萍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院病理研究所,武汉430030 [2]中国人民解放军第457医院三病区,武汉430012

出　　处：《中国组织化学与细胞化学杂志》2011年第2期135-139,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的检测核苷酸切除修复(NER)系统的ERCC-1和RRM-1在非小细胞肺癌(NSCLC)组织中的表达并分析其表达的病理临床意义,为探索NSCLC的有关发生机制及术后辅助化疗提供有用的实验依据。方法应用免疫组织化学二步法检测128例NSCLC组织中ERCC-1和RRM-1的表达,以10例支气管扩张症肺组织作为对照。结果1.ERCC-1在NSCLC组织中的总体阳性表达率为44.53%(57/128),在对照组肺组织气道上皮的阳性表达率为100%(10/10),ERCC-1在NSCLC组织和对照组的阳性率差异有统计学意义(P<0.05);RRM-1在NSCLC组织中的总体阳性表达率为75%(96/128),在对照组肺组织气道上皮不表达,RRM-1在NSCLC组织和对照组的阳性率差异有统计学意义(P<0.05)。2.ERCC-1的表达与NSCLC患者的年龄、组织学类型及分化程度存在相关性(P<0.05);RRM-1的表达与NSCLC患者的年龄存在相关性(P<0.05)。NSCLC组织中ERCC-1与RRM-1的表达呈正相关(P<0.01)。结论ERCC-1表达降低或缺失可能与NSCLC发生有关;NSCLC组织中存在着ERCC-1与RRM-1的差异表达,提示不同个体的NSCLC其生物学行为可能不同,因此检测NSCLC组织中NER系统的表达情况对选择NSCLC的个性化辅助化疗及估计患者的预后有指导意义。Objective To investigate the expression of ERCC-1 and RRM-1 proteins of the nucleotide excision repair（NER） system in patients with non-small cell lung cancer（NSCLC） and to analyze their correlations with the clinicopathological characteristics,providing a helpful experimental evidence for exploring NSCLC pathogenesis and resistance to chemotherapeutic drugs.Methods Immunohistochemical methods were used to investigate the expression of ERCC-1 and RRM-1 proteins in 128 cases of histopathologically defined NSCLC and 10 cases of bronchiectasis.Results 1.The positive expression rate of ERCC-1 and RRM-1 in NSCLC was 44.53%（57/128） and 75%（96/128）,respectively.The different positive rates between NSCLC and control lung tissues had statistical significance.2.The expression of ERCC-1 was related to age,histological type and tumor grade,but the expression of RRM-1 was related to age in patients with NSCLC.The relationship between ERCC-1 and RRM-1 was positive.Conclusion The reduction or lack of ERCC-1 expression may be significant in the pathogenesis of NSCLC.The expressions of ERCC-1 and RRM-1 were different in some individuals of NSCLC,indicating that the biological behavior may be different in them.It may be of great significance to detect the expression of the NER system in NSCLC for choosing the best adjuvant chemotherapy and estimating individual patient＇s prognosis.

关 键 词：错配切除交叉互补修复酶1 核糖核苷酸还原酶M1 非小细胞肺癌 

分 类 号：R365[医药卫生—病理学]