烟草中蔗糖四酯类化合物的GC-MS分析  

Analysis of sucrose tetra-esters from tobaccos by GC-MS

作　　者：王瑞玲[1] 王莹莹[1] 毛多斌[2] 贾春晓[1] 

机构地区：[1]郑州轻工业学院材料与化学工程学院,河南郑州450002 [2]郑州轻工业学院食品与生物工程学院,河南郑州450002

出　　处：《化学研究与应用》2011年第8期1030-1035,共6页Chemical Research and Application

基　　金：国家烟草局重大科技专项(2009ZX09306-001-5)资助

摘　　要：采用超声波辅助溶剂萃取技术,用GC-MS联用法对烟草(香料烟、烤烟和卷烟)中的蔗糖四酯类化合物(STE)进行分析。烟样中的STE用响应面法优化后的最佳萃取条件进行萃取;所得萃取物经硅醚衍生化后用GC-MS方法分析,以内标标准曲线法测定其中STE的含量;并对19种不同产地、品种和等级的烟样进行了分析。结果表明,该方法的平均回收率在98.53%～101.49%之间,线性相关系数大于0.999,相对标准偏差小于2.9%,方法检出限在0.14～0.17μg/mL之间。香料烟中STE含量较高,鉴定出STE Ⅰ～STE Ⅴ共5种蔗糖四酯,其次是卷烟,主要含STE Ⅰ和STE Ⅱ,而烤烟中STE含量最少,主要含STE Ⅰ、STE Ⅱ和STE Ⅳ。A method for the analysis of sucrose tetra-ester（STE）in ultrasonic–assisted extraction products from tobaccos by gas chromatography-mass spectrometry（GC-MS）was developed.The STE were extracted under the optimal extraction conditions which optimized by the response surface methodology,the STE derived with N,O-bis（trimethylsilyl）trifluoroacetamide and analyzed by GC-MS method.The contents of STE in tobaccos were determined by internal standard curve method and 19 kinds of tobacco were analyzed.The results showed that the average added standard recovery of the method developed by this paper was in the range of 98.53%～101.49%,the relative standard deviations（RSD）were less than 2.9%,the correlation coefficients were more than 0.999 and the detection limits were in the range of 0.14～0.17μg/mL.5 kinds of STE（STEⅠ～SE Ⅵ）were identified from oriental tobacco,3 kinds of STE（STEⅠ,SE II and SE IV）were identified from flue-cured and 2 kinds of STE（STE I and SE II）were identified from cigarette.The total content of STE in oriental tobacco was higher than that in flue-cured and cigarette,and flue-cured has the least content of STE.

关 键 词：气相色谱-质谱联用 烟草 蔗糖四酯 

分 类 号：O657.7[理学—分析化学]