不同脂肪酸构成对大鼠肝脏HMG-CoAR、SREBP-1c表达的影响  

Effects of different dietary fatty acid composition on HMG-CoAR and SREBP-1c expression in rat liver

作　　者：时皎皎[1] 糜漫天[2] 韦娜[2] 王斌[2] 

机构地区：[1]成都军区总医院营养科,成都610083 [2]第三军医大学营养与食品卫生学教研室重庆市营养与食品安全重点实验室

出　　处：《西南国防医药》2011年第8期813-816,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：国家自然科学基金资助项目(30500408);"达能"营养基金资助项目(2004)

摘　　要：目的探讨不同膳食脂肪酸构成比对大鼠脂质代谢相关基因HMG-CoAR、SREBP-1c的mRNA表达的影响。方法雌性SD大鼠随机分成6组,喂养6种不同膳食脂肪酸组成的饲料,即饱和脂肪酸组(SFA)、单不饱和脂肪酸组(MUFA)、n-6多不饱和脂肪酸组(n-6 PUFA)、n-3多不饱和脂肪酸组(n-3 PUFA)、1:1 n-6/n-3组及普通饲料喂养组,持续喂养18 w。并在实验第8 w和18 w时,提取大鼠肝脏组织,利用RT-PCR技术检测大鼠肝脏组织中HMG-CoAR、SREBP-1c mRNA表达状况。结果与对照组相比较,SFA能非常显著地升高HMG-CoAR mRNA的表达(P<0.01),MUFA能显著降低HMG-CoAR的基因表达(P<0.05),而PUFA则能够显著降低HMG-CoAR和SREBP-1c的基因表达(P<0.05)。且在相同的总脂肪含量下,n-3 PUFA和n-6/n-3配比的膳食干预效果优于n-6 PUFA。结论 SFA、MUFA主要是通过HMG-CoAR途径来调控机体的脂质代谢,它们对SREBP-1c的表达无明显影响。PUFA调节血脂的作用机制可能与脂代谢基因HMG-CoAR和SREBP-1c有关。Objective To study the effects of different dietary fatty acid composition on the mRNA expression of two rat lipid metabolism-related genes-HMG-CoAR and SREBP-1c.Methods 48 female rats were randomly divided into six groups and respectively fed with six diets containing different fatty acid for 18 w as follow： saturated fatty acid（SFA）,monounsaturated fatty acid（MUFA）,n-6 polyunsaturated fatty acid（n-6 PUFA）,n-3 PUFA,1∶ 1 n-6/n-3 PUFA and general diet（control group）.After feeding for 8 and 18 w,liver tissues of animals were obtained to detect the mRNA expression of HMG-CoAR and SREBP-1c using RT-PCR.Results Compared with general diet,SFA could increase the mRNA expression of HMG-CoAR very significantly（P0.01）,while MUFA could decrease it significantly（P0.05）.Both mRNA expression of HMG-CoAR and SREBP-1c deceased significantly in the three PUFA groups compared with control group（P0.05 or P0.01）.SREBP-1c expression dropped more obviously in n-3 PUFA and n-6/n-3 PUFA groups than that in n-6 PUFA group（P0.01）.Moreover,n-3 PUFA or n-6/n-3 PUFA had better lipid-lowering effect than n-6 PUFA when the total amount of lipid was similar.Conclusion SFA and MUFA appear to regulate body lipid metabolism mainly through HMG-CoAR path.They have little impact on SREBP-1c expression.However,PUFA may regulate body lipid metabolism through both HMG-CoAR path and SREBP-1c path.

关 键 词：脂肪酸 肝脏 膳食 HMG-CoAR SREBP-1C 大鼠 

分 类 号：R589.2[医药卫生—内分泌]