RNAi沉默HIF-1α对人宫颈癌细胞株辐射敏感性的影响  

THE EFFECT OF SILENCING HIF-1Α ON RADIOSENSITIVITY IN HUMAN CERVICAL CARCINOMA CELL LINE

作　　者：李晓愚[1] 刘方欣[2] 任庆兰[3] 李少林[1] 

机构地区：[1]重庆医科大学放射医学教研室,重庆400016 [2]重庆医科大学实验管理中心 [3]重庆医科大学附属第一医院肿瘤科

出　　处：《现代预防医学》2011年第16期3285-3287,共3页Modern Preventive Medicine

摘　　要：[目的]探讨在乏氧条件下,应用RNA干扰技术沉默HIF-1α基因表达对人宫颈癌细胞株U14辐射敏感性的影响。[方法]构建针对HIF-1α基因的siRNA,将其结合到pSilencer2.1-U6-neo质粒表达载体上,脂质体转染法转入宫颈癌细胞U14,低氧培养后RT-PCR检测HIF-1αmRNA的表达;成克隆实验检测HIF-1α沉默后对细胞辐射敏感性的影响。[结果]成功构建HIF-1α的siRNA真核表达载体,转染宫颈癌细胞后低氧条件下RT-PCR显示HIF-1αmRNA表达明显下调,同时细胞形成的克隆数明显减少。[结论]HIF-1α的siRNA真核表达载体能够抑制低氧条件下HIF-1α基因的表达并提高宫颈癌细胞对γ射线的辐射敏感性。[Objective]To study the effect of silencing HIF-1α on radiosensitivity in human cervical carcinoma cell line under hypoxia.[Methods]HIF-1α specific RNAi was bonded to pSilencer2.1-U6-neo plasmid vector and transfected to u14 cell. RT-PCR analysis was used to detect the expression of HIF-1α mRNA. Clonogenic assay was employed to observe the effects on the radiosensitivity after HIF-1α silencing.[Results]The HIF-1α specific RNAi plasmid vector was constructed successfully. The expression of HIF-1α mRNA and the clone of U14 cell were increased after HIF-1α silencing.[Conclusion]The HIF-1α specific RNAi plasmid vector can downregulate the expression of HIF-1α mRNA and enhance the radiosensitivity of cervical carcinoma cell line in vivo.

关 键 词：宫颈癌 RNA干扰 低氧诱导因子-1Α 辐射敏感性 

分 类 号：R737.33[医药卫生—肿瘤]