内源性NO对裸鼠肝癌模型中HDAC4表达的影响  

Effect of endogenous NO on expression of HDAC4 in a hepatocellular carcinoma model of nude mice

作　　者：许诺[1] 姜军梅[1] 冯珊珊[1] 王冠华[1] 孟玫[1] 尹晓燕[1] 

机构地区：[1]山东大学附属省立医院消化内科,济南250021

出　　处：《山东大学学报（医学版）》2011年第8期48-51,共4页Journal of Shandong University:Health Sciences

基　　金：山东省优秀青年科学家奖励基金资助项目(2000BB2DBA);山东省科技攻关基金资助项目(2005GG4402049)

摘　　要：目的观察内源性一氧化氮(nitric ox ide,NO)对裸鼠肝癌模型中组蛋白去乙酰化酶4(histone deacety lase4,HDAC4)表达的影响,从而探讨新的抗肿瘤思路。方法免疫组化方法检测已经建立好的裸鼠肝癌模型中对照组、5-氟尿嘧啶组(5-FU)、5-氟尿嘧啶+L-精氨酸组(5-FU+L-A rg)诱导型一氧化氮合酶(inducib le nitric ox idesynthase,iNO S)与HDAC4的表达情况。结果 iNO S在对照组、5-FU组、5-FU+L-A rg组中表达呈平行递增关系,而HDAC4则表达为下降关系,每两组间比较差异均有统计学意义(P<0.05),iNO S和HDAC4在5-FU组、5-FU+L-A rg组的表达有显著相关性(P<0.05)。结论内源性NO下调HDAC4的表达,是HDAC功能作用的重要调节者,为抗肿瘤研究提供新的思路。Objective To investigate the effect of endogenous NO on expression of histone deacetylase 4 （HDAC4） in a hepatocellular carcinoma model of nude mice, and to explore a new antitumor idea. Methods Expressions of inducible nitric oxide synthase（iNOS） and HDAC4 were detected by immunohistochemistry in the control group, the 5-FU group and the 5-FU ＋ L-Arg group in the established hepatocellular carcinoma model of nude mice. Results Expression of iNOS in the control group, the 5-FU group and the 5-FU ＋ L-Arg group was increased, while expression of HDAC4 was depressed. Differences between the two groups were significant（ P 〈 0.05 ）. Expressions of iNOS and HDAC4 in the 5-FU group and 5-FU ＋ L-Arg group had a significant correlation. （ P 〈 0.05 ）. Conclusion Endogenous NO down-regulates expression of HDAC4, which is a key regulator of multiple HDAC functions. The relationship between endogenous NO and HDAC4 provides a new idea for antitumor study.

关 键 词：肝癌 一氧化氮 一氧化氮合酶 组蛋白去乙酰化酶 

分 类 号：R735.7[医药卫生—肿瘤]