基于大孔树脂吸附的樟芝胞外总三萜的分离工艺  

Separation of Total Triterpenoids from Culture Broth of Antrodia camphorata Based on Macroporous Resin Adsorption

作　　者：陆震鸣 许泓瑜 史劲松 许正宏 

机构地区：[1]江南大学医药学院制药工程研究室,江苏无锡214122 [2]江南大学医药学院生物活性制品加工工程研究室,江苏无锡214122

出　　处：《食品与发酵工业》2011年第6期188-192,共5页Food and Fermentation Industries

基　　金：江苏省自然科学基金(BK2010142);教育部新世纪人才支持计划(No.NCET-07-0380);国家"863"重点项目子课题(No.2007AA021506)

摘　　要：以总三萜的洗脱效果为考察指标,研究了大孔树脂分离纯化的吸附性能及洗脱参数。结果表明,XAD-16大孔树脂适宜樟芝胞外总三萜的富集分离,其再生能力良好,吸附过程符合Langmuir方程。同时获得了XAD-16大孔树脂对三萜类化合物的动态分离条件:上样发酵液的pH为6.0,吸附流速为2 mL/min,解吸附溶剂为乙醇。溶液中三萜类化合物的初始浓度为0.97 mg/mL,获得了纯度为260.54 mg/g的总三萜样品。Based on desorption effect on total triterpenoids in culture broth of Antrodia camphorata,the adsorption capability and desorption parameters of macroporous resin were studied.The best macroporous resin,XAD-16,has good regeneration ability and the adsorption process is in accordance with Langmuir equation.The optimal separation conditions were as follows： culture broth with pH of 6.0,flow rate of 2 mL/min,100% Ethanol as desorption solution.After separation by macroporous resin,total triterpenoids sample with the purity of 260.54 mg/g was obtained from culture broth of Antrodia camphorata.

关 键 词：大孔树脂 总三萜 樟芝 

分 类 号：TQ461[化学工程—制药化工]